Evidence map›Paper›PMID 29732089›Full record

ArticleChemical science2018

Aligner-mediated cleavage of nucleic acids and its application to isothermal exponential amplification.

Wanghua Wu, Tao Zhang, Da Han, Hongliang Fan, Guizhi Zhu, Xiong Ding, Cuichen Wu, Mingxu You, Liping Qiu, Juan Li and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Aptamer Blocking Strategy Inhibits SARS-CoV-2 Virus Infection.Angewandte Chemie (International ed. in English) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Wanghua WuResearch Center for Analytical Instrumentation , Institute of Cyber-Systems and Control , State Key Laboratory of Industrial Control Technology , Zhejiang University , Hangzhou 310027 , China . Email: zhtao@zju.edu.cn.
Tao ZhangResearch Center for Analytical Instrumentation , Institute of Cyber-Systems and Control , State Key Laboratory of Industrial Control Technology , Zhejiang University , Hangzhou 310027 , China . Email: zhtao@zju.edu.cn.ORCID 0000-0003-3782-0478
Da HanCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Hongliang FanResearch Center for Analytical Instrumentation , Institute of Cyber-Systems and Control , State Key Laboratory of Industrial Control Technology , Zhejiang University , Hangzhou 310027 , China . Email: zhtao@zju.edu.cn.
Guizhi ZhuCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Xiong DingResearch Center for Analytical Instrumentation , Institute of Cyber-Systems and Control , State Key Laboratory of Industrial Control Technology , Zhejiang University , Hangzhou 310027 , China . Email: zhtao@zju.edu.cn.
Cuichen WuCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Mingxu YouCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Liping QiuCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Juan LiCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Liqin ZhangCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.ORCID 0000-0002-3632-4117
Xiang LianResearch Center for Analytical Instrumentation , Institute of Cyber-Systems and Control , State Key Laboratory of Industrial Control Technology , Zhejiang University , Hangzhou 310027 , China . Email: zhtao@zju.edu.cn.
Rong HuCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.ORCID 0000-0002-0009-2473
Ying MuResearch Center for Analytical Instrumentation , Institute of Cyber-Systems and Control , State Key Laboratory of Industrial Control Technology , Zhejiang University , Hangzhou 310027 , China . Email: zhtao@zju.edu.cn.
Jianguang ZhouResearch Center for Analytical Instrumentation , Institute of Cyber-Systems and Control , State Key Laboratory of Industrial Control Technology , Zhejiang University , Hangzhou 310027 , China . Email: zhtao@zju.edu.cn.
Weihong TanCenter for Research at Bio/nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute and McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.ORCID 0000-0002-8066-1524

Funding

Development of molecular probes for biomedical applicationsR01GM079359 · NIGMS · UNIVERSITY OF FLORIDA · PI TAN, WEIHONG · 2007 to 2016
$2.3M
NIGMS NIH HHS R01 GM079359
6 · The paper itself

Abstract

We herein describe a simple and versatile approach to use conventional nicking endonuclease (NEase) for programmable sequence-specific cleavage of DNA, termed aligner-mediated cleavage (AMC), and its application to DNA isothermal exponential amplification (AMC-based strand displacement amplification, AMC-SDA). AMC uses a hairpin-shaped DNA aligner (DA) that contains a recognition site in its stem and two side arms complementary to target DNA. Thus, it enables the loading of an NEase on DA's stem, localization to a specific locus through hybridization of the side arms with target DNA, and cleavage thereof. By using just one NEase, it is easy to make a break at any specific locus and tune the cleavage site to the single-nucleotide scale. This capability also endows the proposed AMC-SDA with excellent universality, since the cleavage of target DNA, followed by a polymerase-catalyzed extension along a particular primer as a key step for initiating SDA, no longer relies on any special sequence. Moreover, this manner of initiation facilitates the adoption of 3'-terminated primers, thus making AMC-SDA highly sensitive and highly specific, as well as simple primer design.

Identifiers

PMID29732089
PMCPMC5916018

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.