ArticleSensors and actuators. B, Chemical2018
Electrical impedance microflow cytometry with oxygen control for detection of sickle cells.
Article in Sensors and actuators. B, Chemical, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 60 citations in OpenAlex.
- Acoustic probing of new biomarkers for rapid sickle cell disease screening.Lab on a chip · 2026Article
- In situ detection of dead cells from live cells via a DC plus low frequency AC resistive pulse sensor.Biomedical microdevices · 2026Article
- Review
- A Review on Microfluidics-Based Impedance Biosensors.Biosensors · 2023Review
- Label-free testing strategy to evaluate packed red blood cell quality before transfusion to leukemia patients.Scientific reports · 2022Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis.Biosensors · 2021Review
- Electrical Impedance of Upper Limb Enables Robust Wearable Identity Recognition against Variation in Finger Placement and Environmental Factors.Biosensors · 2021Article
- A portable impedance microflow cytometer for measuring cellular response to hypoxia.Biotechnology and bioengineering · 2021Article
- Techniques for the Detection of Sickle Cell Disease: A Review.Micromachines · 2021Review
- Microfluidic electrical impedance assessment of red blood cell-mediated microvascular occlusion.Lab on a chip · 2021Article
- Smartphone-based sickle cell disease detection and monitoring for point-of-care settings.Biosensors & bioelectronics · 2020Article
- Biosensors for Detection of Human Placental Pathologies: A Review of Emerging Technologies and Current Trends.Translational research : the journal of laboratory and clinical medicine · 2019Review
- Use of an Insulation Layer on the Connection Tracks of a Biosensor with Coplanar Electrodes to Increase the Normalized Impedance Variation.Biosensors · 2019Article
- Electrical Impedance Characterization of Erythrocyte Response to Cyclic Hypoxia in Sickle Cell Disease.ACS sensors · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Polymerization of intracellular sickle hemoglobin induced by low oxygen tension has been recognized as a primary determinant of the pathophysiologic manifestations in sickle cell disease. Existing flow cytometry techniques for detection of sickle cells are typically based on fluorescence markers or cellular morphological analysis. Using microfluidics and electrical impedance spectroscopy, we develop a new, label-free flow cytometry for non-invasive measurement of single cells under controlled oxygen level. We demonstrate the capability of this new technique by determining the electrical impedance differential of normal red blood cells obtained from a healthy donor and sickle cells obtained from three sickle cell patients, under normoxic and hypoxic conditions and at three different electrical frequencies, 156 kHz, 500 kHz and 3 MHz. Under normoxia, normal cells and sickle cells can be separated completely using electrical impedance at 156 kHz and 500 kHz but not at 3 MHz. Sickle cells, intra-patient and inter-patient show significantly different electrical impedance between normoxia and hypoxia at all three frequencies. This study shows a proof of concept that electrical impedance signal can be used as an indicator of the disease state of a red blood cell as well as the cell sickling events in sickle cell disease. Electrical impedance-based microflow cytometry with oxygen control is a new method that can be potentially used for sickle cell disease diagnosis and monitoring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.