ArticleCurrent biology : CB2018
Quantitative Control of GPCR Organization and Signaling by Endocytosis in Epithelial Morphogenesis.
Article in Current biology : CB, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Spatial patterning of contractility by a self-organized mechanogen activity gradient underlies Drosophila gastrulation.Nature communications · 2026Article
- Article
- Actomyosin pulsing rescues embryonic tissue folding from disruption by myosin fluctuations.Research square · 2023Article
- Serotonin signaling regulates actomyosin contractility during morphogenesis in evolutionarily divergent lineages.Nature communications · 2023Article
- Membrane-actin interactions in morphogenesis: Lessons learned from Drosophila cellularization.Seminars in cell & developmental biology · 2023Review
- Interplay between mechanics and signalling in regulating cell fate.Nature reviews. Molecular cell biology · 2022Review
- Actomyosin activity-dependent apical targeting of Rab11 vesicles reinforces apical constriction.The Journal of cell biology · 2022Article
- DAPLE orchestrates apical actomyosin assembly from junctional polarity complexes.The Journal of cell biology · 2022Article
- Endocytosis at the Crossroad of Polarity and Signaling Regulation: Learning fromInternational journal of molecular sciences · 2022Review
- Orthogonal Peptide-Templated Labeling Elucidates Lateral ETChembiochem : a European journal of chemical biology · 2022Article
- Endocytosis in the context-dependent regulation of individual and collective cell properties.Nature reviews. Molecular cell biology · 2021Review
- Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry.Developmental cell · 2021Article
- Combinatorial patterns of graded RhoA activation and uniform F-actin depletion promote tissue curvature.Development (Cambridge, England) · 2021Article
- Regulation of apical constriction via microtubule- and Rab11-dependent apical transport during tissue invagination.Molecular biology of the cell · 2021Article
- Distinct actin-dependent nanoscale assemblies underlie the dynamic and hierarchical organization of E-cadherin.Current biology : CB · 2021Article
- FGFR/Heartless and Smog interact synergistically to negatively regulate Fog mediated G-protein coupled receptor signaling in the Drosophila nervous system.G3 (Bethesda, Md.) · 2021Article
- TheMolecular biology of the cell · 2020Article
- Orchestrating morphogenesis: building the body plan by cell shape changes and movements.Development (Cambridge, England) · 2020Review
- The pulse of morphogenesis: actomyosin dynamics and regulation in epithelia.Development (Cambridge, England) · 2020Review
- Cellular processes driving gastrulation in the avian embryo.Mechanisms of development · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Tissue morphogenesis arises from controlled cell deformations in response to cellular contractility. During Drosophila gastrulation, apical activation of the actomyosin networks drives apical constriction in the invaginating mesoderm and cell-cell intercalation in the extending ectoderm. Myosin II (MyoII) is activated by cell-surface G protein-coupled receptors (GPCRs), such as Smog and Mist, that activate G proteins, the small GTPase Rho1, and the kinase Rok. Quantitative control over GPCR and Rho1 activation underlies differences in deformation of mesoderm and ectoderm cells. We show that GPCR Smog activity is concentrated on two different apical plasma membrane compartments, i.e., the surface and plasma membrane invaginations. Using fluorescence correlation spectroscopy, we probe the surface of the plasma membrane, and we show that Smog homo-clusters in response to its activating ligand Fog. Endocytosis of Smog is regulated by the kinase Gprk2 and β-arrestin-2 that clears active Smog from the plasma membrane. When Fog concentration is high or endocytosis is low, Smog rearranges in homo-clusters and accumulates in plasma membrane invaginations that are hubs for Rho1 activation. Lastly, we find higher Smog homo-cluster concentration and numerous apical plasma membrane invaginations in the mesoderm compared to the ectoderm, indicative of reduced endocytosis. We identify that dynamic partitioning of active Smog at the surface of the plasma membrane or plasma membrane invaginations has a direct impact on Rho1 signaling. Plasma membrane invaginations accumulate high Rho1-guanosine triphosphate (GTP) suggesting they form signaling centers. Thus, Fog concentration and Smog endocytosis form coupled regulatory processes that regulate differential Rho1 and MyoII activation in the Drosophila embryo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.