ArticleMicrobial cell factories2018
Metabolic engineering of Saccharomyces cerevisiae for efficient production of glucaric acid at high titer.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 86 citations in OpenAlex.
- The visualizable and marker-free gene editing platform mediated by CRISPR/Cas9 in Coleophoma empetri.Applied microbiology and biotechnology · 2026Article
- Microbial Bio-refineries: Engineering Microbes for the Production of Versatile Short-Chain Organic Acids.Indian journal of microbiology · 2025Review
- Metabolic engineering for microbial production of sugar acids.BMC biotechnology · 2025Review
- 4-O-Methylglucaric Acid Production from Xylan with Uronic Acid Oxidase and Comparison to Glucaric Acid from Glucose.Chembiochem : a European journal of chemical biology · 2025Article
- Cell factories for biosynthesis of D-glucaric acid: a fusion of static and dynamic strategies.World journal of microbiology & biotechnology · 2024Review
- An Eclectic Review on Dicarboxylic Acid Production Through Yeast Cell Factories and Its Industrial Prominence.Current microbiology · 2024Review
- Production of D-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae.Biotechnology letters · 2024Article
- Development of a Novel D-Lactic Acid Production Platform Based on Lactobacillus saerimneri TBRC 5746.Journal of microbiology (Seoul, Korea) · 2023Article
- Efficient Production of Glucaric Acid by Engineered Saccharomyces cerevisiae.Applied and environmental microbiology · 2023Article
- Engineering Flocculation for Improved Tolerance and Production of d-Lactic Acid inJournal of fungi (Basel, Switzerland) · 2023Article
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Improving yields by switching central metal ions in porphyrazine-catalyzed oxidation of glucose into value-added organic acids with SnOFrontiers in chemistry · 2023Article
- Developing Multi-Copy Chromosomal Integration Strategies for Heterologous Biosynthesis of Caffeic Acid inFrontiers in microbiology · 2022Article
- Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast.ACS synthetic biology · 2021Article
- Implementation of Synthetic Pathways to Foster Microbe-Based Production of Non-Naturally Occurring Carboxylic Acids and Derivatives.Journal of fungi (Basel, Switzerland) · 2021Review
- Screening neutral sites for metabolic engineering of methylotrophic yeastSynthetic and systems biotechnology · 2021Article
- Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium.Biotechnology for biofuels · 2021Article
- Cell-based and cell-free biocatalysis for the production of D-glucaric acid.Biotechnology for biofuels · 2020Review
- Enzymatic production of 4-Biotechnology for biofuels · 2020Article
- Transcription Factor Engineering for High-Throughput Strain Evolution and Organic Acid Bioproduction: A Review.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundGlucaric acid is a high-value-added chemical that can be used in various fields. Because chemical oxidation of glucose to produce glucaric acid is not environmentally friendly, microbial production has attracted increasing interest recently. Biological pathways to synthesize glucaric acid from glucose in both Escherichia coli and Saccharomyces cerevisiae by co-expression of genes encoding myo-inositol-1-phosphate synthase (Ino1), myo-inositol oxygenase (MIOX), and uronate dehydrogenase (Udh) have been constructed. However, low activity and instability of MIOX from Mus musculus was proved to be the bottleneck in this pathway.
resultsA more stable miox4 from Arabidopsis thaliana was chosen in the present study. In addition, high copy delta-sequence integration of miox4 into the S. cerevisiae genome was performed to increase its expression level further. Enzymatic assay and quantitative real-time PCR analysis revealed that delta-sequence-based integrative expression increased MIOX4 activity and stability, thus increasing glucaric acid titer about eight times over that of episomal expression. By fed-batch fermentation supplemented with 60 mM (10.8 g/L) inositol, the multi-copy integrative expression S. cerevisiae strain produced 6 g/L (28.6 mM) glucaric acid from myo-inositol, the highest titer that had been ever reported in S. cerevisiae.
conclusionsIn this study, glucaric acid titer was increased to 6 g/L in S. cerevisiae by integrating the miox4 gene from A. thaliana and the udh gene from Pseudomonas syringae into the delta sequence of genomes. Delta-sequence-based integrative expression increased both the number of target gene copies and their stabilities. This approach could be used for a wide range of metabolic pathway engineering applications with S. cerevisiae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.