Evidence map›Paper›PMID 29728894›Full record

ArticleJournal of molecular histology2018

Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.

Lawrence Owusu, Caiming Xu, Hailong Chen, Geliang Liu, Guixin Zhang, Jinwen Zhang, Zhankai Tang, Zhongwei Sun, Xin Yi

Abstract read
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In one paragraph

Article in Journal of molecular histology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Lawrence OwusuDepartment of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian, 116011, Liaoning Province, China.
Caiming XuDepartment of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian, 116011, Liaoning Province, China.
Hailong ChenDepartment of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian, 116011, Liaoning Province, China. chenhailong2007@yahoo.com.ORCID http://orcid.org/0000-0001-5247-7343
Geliang LiuDepartment of Surgery, The Second Affiliated Hospital, Dalian Medical University, Dalian, 116023, Liaoning Province, China.
Guixin ZhangDepartment of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian, 116011, Liaoning Province, China.
Jinwen ZhangDepartment of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian, 116011, Liaoning Province, China.
Zhankai TangDepartment of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian, 116011, Liaoning Province, China.
Zhongwei SunDepartment of Surgery, The First Affiliated Hospital, Dalian Medical University, No. 222 Zhongshan Road, Dalian, 116011, Liaoning Province, China.
Xin YiDepartment of Biotechnology, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Dalian Medical University · CN

Funding

National Natural Science Foundation of China 81573751
6 · The paper itself

Abstract

Severe acute pancreatitis (SAP) associated acute lung injury (ALI) accounts for about 70% mortality of SAP patients. However, there are no precise biomarkers for the disease currently. Herein, we evaluated the potential of gamma-enolase (ENO2), against its universal isoform alpha-enolase (ENO1), as a marker of SAP-ALI in a rat model. Firstly, 16 male Sprague-Dawley rats were randomly divided into two groups, Sham (n = 8) and SAP-ALI (n = 8), for pancreatitis induction. Ultra-structure examination by electron microscopy and HE staining were used for lung injury assessment. Lung tissue expressions of alpha-enolase and gamma-enolase were evaluated by qRT-PCR and immunohistochemistry. In a prospective validation experiment, 28 rats were used: sham (n = 8), SAP-ALI at 3 h (3 h, n = 10), and SAP-ALI at 24 h (24 h, n = 10). Lung tissue damage, tissue expression and circulating alpha-enolase and gamma-enolase levels were evaluated. Elevated serum levels of α-amylase and TNF-α were observed in SAP rats but not in sham-operated rats. Histological examination of pancreatic and lung tissues indicated marked damage in SAP rats. While alpha-enolase was universally expressed, gamma-enolase was expressed only in damaged lung tissues. Gamma-enolase was detected in lung tissues, BALF, and serum as early as 3 h post-surgery when physical pathological damage was not apparent. Unlike alpha-enolase, secreted and/or circulating gamma-enolase level progressively increased, especially in serum, as lung damage progressed. Thus, gamma-enolase may signal and correlate lung tissue damage well before obvious physical pathological tissue damage and might be a candidate diagnostic and/or prognostic marker.

Indexed as

Acute Lung InjuryAnimalsBiomarkersBronchoalveolar Lavage FluidDisease Models, AnimalLungMalePancreatitisPhosphopyruvate HydrataseRats, Sprague-DawleyReproducibility of ResultsTime FactorsTumor Necrosis Factor-alphaBiomarkersPhosphopyruvate HydrataseTumor Necrosis Factor-alphaAlpha-enolaseGamma-enolaseSevere acute pancreatitis-induced acute lung injurySprague–Dawley rats

Identifiers

PMID29728894
OpenAlexW2799277863

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.