ArticleJournal of cell science2018
Microtubules as platforms for probing liquid-liquid phase separation in cells - application to RNA-binding proteins.
Article in Journal of cell science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Maternal Huluwa regulates postfertilization microtubule array organization for asymmetrical transport of dorsal determinants in zebrafish.Nature communications · 2026Article
- mRNA recruitment by G3BP1 condensates is regulated by Caprin1 but requires G3BP1 binding to mRNA.Scientific reports · 2025Article
- Glutamine modulates stress granule formation in cancer cells through core RNA-binding proteins.Journal of cell science · 2025Article
- TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm.Communications biology · 2025Article
- HuR as a molecular target for cancer therapeutics and immune-related disorders.Advanced drug delivery reviews · 2022Review
- Liquid-liquid phase separation underpins the formation of replication factories in rotaviruses.The EMBO journal · 2021Article
- The cooperative binding of TDP-43 to GU-rich RNA repeats antagonizes TDP-43 aggregation.eLife · 2021Article
- Liquid-liquid phase separation of tau: From molecular biophysics to physiology and disease.Protein science : a publication of the Protein Society · 2021Review
- Lin28, a major translation reprogramming factor, gains access to YB-1-packaged mRNA through its cold-shock domain.Communications biology · 2021Article
- ITSN1 regulates SAM68 solubility through SH3 domain interactions with SAM68 proline-rich motifs.Cellular and molecular life sciences : CMLS · 2021Article
- Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions.Annual review of biophysics · 2020Review
- Emerging Roles of Biomolecular Condensates in Pre-mRNA 3' End Processing.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid-liquid phase separation enables compartmentalization of biomolecules in cells, notably RNA and associated proteins in the nucleus. Besides having critical functions in RNA processing, there is a major interest in deciphering the molecular mechanisms of compartmentalization orchestrated by RNA-binding proteins such as TDP-43 (also known as TARDBP) and FUS because of their link to neuron diseases. However, tools for probing compartmentalization in cells are lacking. Here, we developed a method to analyze the mixing and demixing of two different phases in a cellular context. The principle is the following: RNA-binding proteins are confined on microtubules and quantitative parameters defining their spatial segregation are measured along the microtubule network. Through this approach, we found that four mRNA-binding proteins, HuR (also known as ELAVL1), G3BP1, TDP-43 and FUS form mRNA-rich liquid-like compartments on microtubules. TDP-43 is partly miscible with FUS but immiscible with either HuR or G3BP1. We also demonstrate that mRNA is essential to capture the mixing and demixing behavior of mRNA-binding proteins in cells. Taken together, we show that microtubules can be used as platforms to understand the mechanisms underlying liquid-liquid phase separation and their deregulation in human diseases.
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Registered trials
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