ArticleBMC biotechnology2018
Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis.
Article in BMC biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
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- Chemiluminescent screening of specific hybridoma cells via a proximity-rolling circle activated enzymatic switch.Communications biology · 2022Article
- Optimised expression and purification of RBP4 and preparation of anti-RBP4 monoclonal antibody.FEBS open bio · 2022Article
- A sensitive and rapid method of lead detection using nanoparticle technology based on monoclonal antibody.Frontiers in bioengineering and biotechnology · 2022Article
- Molecular and structural basis of nucleoside diphosphate kinase-mediated regulation of spore and sclerotia development in the fungusThe Journal of biological chemistry · 2019Article
- Four LysR-type transcriptional regulator family proteins (LTTRs) involved in antibiotic resistance in Aeromonas hydrophila.World journal of microbiology & biotechnology · 2019Article
- Generation and characterization of a monoclonal antibody against human BCL6 for immunohistochemical diagnosis.PloS one · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive tumor as a biomarker.
resultsThe synthesized gene of CgA coding area was cloned and expressed as fusion protein CgA-His in procaryotic system. Then the purified CgA-His protein was mixed with QuickAntibody-Mouse5W adjuvant, and injected into mice. The CgA-His protein was also used as coating antigen to determine the antiserum titer. By screening, a stable cell line named 4E5, which can generate anti-CgA monoclonal antibody (mAb), was obtained. The isotype of 4E5 mAb was IgG
conclusionsA sensitive and reliable method was successfully developed for rapid production of anti-CgA mAb for immunohistochemistry diagnosis in this study, and the current study also provides conclusive guidelines for preparation of mAbs and implements in immunohistochemistry diagnosis.
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