ArticleCell2018
Transcriptome-wide Interrogation of the Functional Intronome by Spliceosome Profiling.
Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- DBR1 orchestrates the fate of lariat RNA: debranching-dependent turnover and function.Nucleic acids research · 2025Review
- Decoding protein-RNA interactions using CLIP-based methodologies.Nature reviews. Genetics · 2024Review
- A proteogenomic analysis of cervical cancer reveals therapeutic and biological insights.Nature communications · 2024Article
- Profiling lariat intermediates reveals genetic determinants of early and late co-transcriptional splicing.Molecular cell · 2022Article
- Genome-wide detection of human variants that disrupt intronic branchpoints.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- Article
- Alternative RNA structures formed during transcription depend on elongation rate and modify RNA processing.Molecular cell · 2021Article
- psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation.Nature communications · 2021Article
- Co-transcriptional splicing regulates 3' end cleavage during mammalian erythropoiesis.Molecular cell · 2021Article
- RIPiT-Seq: A tandem immunoprecipitation approach to reveal global binding landscape of multisubunit ribonucleoproteins.Methods in enzymology · 2021Article
- Post-transcriptional splicing of nascent RNA contributes to widespread intron retention in plants.Nature plants · 2020Article
- High throughput approaches to study RNA-protein interactions in vitro.Methods (San Diego, Calif.) · 2020Review
- Splicing Kinetics and Coordination Revealed by Direct Nascent RNA Sequencing through Nanopores.Molecular cell · 2020Article
- A systems view of spliceosomal assembly and branchpoints with iCLIP.Nature structural & molecular biology · 2019Article
- RNA Polymerase II Phosphorylated on CTD Serine 5 Interacts with the Spliceosome during Co-transcriptional Splicing.Molecular cell · 2018Article
- Scrutinizing spliceosomes.Nature reviews. Genetics · 2018Article
- Transcripts from a spliceosome.Nature methods · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Full understanding of eukaryotic transcriptomes and how they respond to different conditions requires deep knowledge of all sites of intron excision. Although RNA sequencing (RNA-seq) provides much of this information, the low abundance of many spliced transcripts (often due to their rapid cytoplasmic decay) limits the ability of RNA-seq alone to reveal the full repertoire of spliced species. Here, we present "spliceosome profiling," a strategy based on deep sequencing of RNAs co-purifying with late-stage spliceosomes. Spliceosome profiling allows for unambiguous mapping of intron ends to single-nucleotide resolution and branchpoint identification at unprecedented depths. Our data reveal hundreds of new introns in S. pombe and numerous others that were previously misannotated. By providing a means to directly interrogate sites of spliceosome assembly and catalysis genome-wide, spliceosome profiling promises to transform our understanding of RNA processing in the nucleus, much as ribosome profiling has transformed our understanding mRNA translation in the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.