ArticleCell2018
Spliceosome Profiling Visualizes Operations of a Dynamic RNP at Nucleotide Resolution.
Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Omics-based dissection of alternative splicing and the regulatory architecture in fungal genomes.Current research in microbial sciences · 2026Review
- CRISPR/Cas9-compatible plasmids enabling seven dominant genetic selection methods for the human fungal pathogenMicrobiology spectrum · 2025Article
- Structures of aberrant spliceosome intermediates on their way to disassembly.Nature structural & molecular biology · 2025Article
- Decoding protein-RNA interactions using CLIP-based methodologies.Nature reviews. Genetics · 2024Review
- GPATCH4 regulates rRNA and snRNA 2'-O-methylation in both DHX15-dependent and DHX15-independent manners.Nucleic acids research · 2024Article
- Intrinsic mesoscale properties of a Polycomb protein underpin heterochromatin fidelity.Nature structural & molecular biology · 2023Article
- Article
- Potential Anti-Tumor Activity of Nardoguaianone L Isolated fromMolecules (Basel, Switzerland) · 2022Article
- Coupling of spliceosome complexity to intron diversity.Current biology : CB · 2021Article
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- Comparative analysis of RNA enrichment methods for preparation of Cryptococcus neoformans RNA sequencing libraries.G3 (Bethesda, Md.) · 2021Article
- Article
- psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation.Nature communications · 2021Article
- Co-transcriptional splicing regulates 3' end cleavage during mammalian erythropoiesis.Molecular cell · 2021Article
- RIPiT-Seq: A tandem immunoprecipitation approach to reveal global binding landscape of multisubunit ribonucleoproteins.Methods in enzymology · 2021Article
- Comprehensive database and evolutionary dynamics of U12-type introns.Nucleic acids research · 2020Article
- Post-transcriptional splicing of nascent RNA contributes to widespread intron retention in plants.Nature plants · 2020Article
- Splicing Kinetics and Coordination Revealed by Direct Nascent RNA Sequencing through Nanopores.Molecular cell · 2020Article
- Ancestral reconstruction of protein interaction networks.PLoS computational biology · 2019Article
- A systems view of spliceosomal assembly and branchpoints with iCLIP.Nature structural & molecular biology · 2019Article
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9 authors.
Funding
Abstract
Tools to understand how the spliceosome functions in vivo have lagged behind advances in the structural biology of the spliceosome. Here, methods are described to globally profile spliceosome-bound pre-mRNA, intermediates, and spliced mRNA at nucleotide resolution. These tools are applied to three yeast species that span 600 million years of evolution. The sensitivity of the approach enables the detection of canonical and non-canonical events, including interrupted, recursive, and nested splicing. This application of statistical modeling uncovers independent roles for the size and position of the intron and the number of introns per transcript in substrate progression through the two catalytic stages. These include species-specific inputs suggestive of spliceosome-transcriptome coevolution. Further investigations reveal the ATP-dependent discard of numerous endogenous substrates after spliceosome assembly in vivo and connect this discard to intron retention, a form of splicing regulation. Spliceosome profiling is a quantitative, generalizable global technology used to investigate an RNP central to eukaryotic gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.