ArticleThe Journal of cell biology1988
Isolated beta-heavy chain subunit of dynein translocates microtubules in vitro.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 98 citations in OpenAlex.
- Force-Generating Mechanism of Axonemal Dynein in Solo and Ensemble.International journal of molecular sciences · 2020Review
- Turning dyneins off bends cilia.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Torque generation by axonemal outer-arm dynein.Biophysical journal · 2015Article
- Distinct roles of 1alpha and 1beta heavy chains of the inner arm dynein I1 of Chlamydomonas flagella.Molecular biology of the cell · 2011Article
- Ratchetlike properties of in vitro microtubule translocation by a Chlamydomonas inner-arm dynein species c in the presence of flow.Biophysical journal · 2009Article
- Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain.Eukaryotic cell · 2008Article
- Mode of Ca2+ action on ciliary beat frequency in single ovine airway epithelial cells.The Journal of physiology · 1999Article
- Evidence for four cytoplasmic dynein heavy chain isoforms in rat testis.Molecular biology of the cell · 1998Article
- Mechanical components of motor enzyme function.Biophysical journal · 1997Article
- Is outer arm dynein intermediate chain 1 multifunctional?Molecular biology of the cell · 1996Article
- Selective adhesion of functional microtubules to patterned silane surfaces.Biophysical journal · 1995Article
- The dynein genes of Paramecium tetraurelia: the structure and expression of the ciliary beta and cytoplasmic heavy chains.Molecular biology of the cell · 1995Article
- Interspecies conservation of outer arm dynein intermediate chain sequences defines two intermediate chain subclasses.Molecular biology of the cell · 1995Article
- A monoclonal antibody against the dynein IC1 peptide of sea urchin spermatozoa inhibits the motility of sea urchin, dinoflagellate, and human flagellar axonemes.Molecular biology of the cell · 1994Article
- Characterization of DLC-A and DLC-B, two families of cytoplasmic dynein light chain subunits.Molecular biology of the cell · 1994Article
- Phylogeny and expression of axonemal and cytoplasmic dynein genes in sea urchins.Molecular biology of the cell · 1994Article
- A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain.The Journal of cell biology · 1993Article
- The motile beta/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond.The Journal of cell biology · 1992Article
- The alpha subunit of sea urchin sperm outer arm dynein mediates structural and rigor binding to microtubules.The Journal of cell biology · 1992Article
- Structural and functional reconstitution of inner dynein arms in Chlamydomonas flagellar axonemes.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Our goal was to assess the microtubule translocating ability of individual ATPase subunits of outer arm dynein. Solubilized outer arm dynein from sea urchin sperm (Stronglocentrotus purpuratus) was dissociated into subunits by low ionic strength buffer and fractionated by zonal centrifugation. Fractions were assessed by an in vitro functional assay wherein microtubules move across a glass surface to which isolated dynein fractions had been absorbed. Microtubule gliding activity was coincident with the 12-S beta-heavy chain-intermediate chain 1 ATPase fractions (beta/IC1). Neither the alpha-heavy chain nor the intermediate chains 2 and 3 fractions coincided with microtubule gliding activity. The beta/IC1 ATPase induced very rapid gliding velocities (9.7 +/- 0.88 micron/s, range 7-11.5 micron/s) in 1 mM ATP-containing motility buffers. In direct comparison, isolated intact 21-S outer arm dynein, from which the beta/IC1 fraction was derived, induced slower microtubule gliding rates (21-S dynein, 5.6 +/- 0.7 micron/s; beta/IC1, 8.7 +/- 1.2 micron/s). These results demonstrate that a single subdomain in dynein, the beta/IC1 ATPase, is sufficient for microtubule sliding activity.
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