ReviewFEMS yeast research2018
Design principles for nuclease-deficient CRISPR-based transcriptional regulators.
Review in FEMS yeast research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- UniClo: scarless hierarchical DNA assembly without sequence constraint.Nucleic acids research · 2025Article
- dCas9-HDAC8-EGFP fusion enables epigenetic editing of breast cancer cells by H3K9 deacetylation.European journal of cell biology · 2024Article
- CRISPRi-induced transcriptional regulation of IAH1 gene and its influence on volatile compounds profile in Kluyveromyces marxianus DU3.World journal of microbiology & biotechnology · 2024Article
- The expanded CRISPR toolbox for constructing microbial cell factories.Trends in biotechnology · 2024Review
- Development and applications of a CRISPR activation system for facile genetic overexpression in Candida albicans.G3 (Bethesda, Md.) · 2023Article
- Building a pipeline to identify and engineer constitutive and repressible promoters.Quantitative plant biology · 2023Article
- Engineering of Synthetic Transcriptional Switches in Yeast.Life (Basel, Switzerland) · 2022Review
- A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast.BMC genomics · 2021Article
- Rational gRNA design based on transcription factor binding data.Synthetic biology (Oxford, England) · 2021Article
- Applications of CRISPR/Cas9 in the Synthesis of Secondary Metabolites in Filamentous Fungi.Frontiers in microbiology · 2021Review
- Multiplex Genome Editing in Yeast by CRISPR/Cas9 - A Potent and Agile Tool to Reconstruct Complex Metabolic Pathways.Frontiers in plant science · 2021Review
- A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12.Scientific reports · 2020Article
- CRISPR-Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference.Nucleic acids research · 2020Article
- Article
- Advances in Sphingolipidoses: CRISPR-Cas9 Editing as an Option for Modelling and Therapy.International journal of molecular sciences · 2019Review
- Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization.Cellular and molecular bioengineering · 2019Article
- Transcription of Bacterial Chromatin.Journal of molecular biology · 2019Review
- Multiplex genome editing of microorganisms using CRISPR-Cas.FEMS microbiology letters · 2019Review
- Engineering metabolite-responsive transcriptional factors to sense small molecules in eukaryotes: current state and perspectives.Microbial cell factories · 2019Review
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The engineering of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated proteins continues to expand the toolkit available for genome editing, reprogramming gene regulation, genome visualisation and epigenetic studies of living organisms. In this review, the emerging design principles on the use of nuclease-deficient CRISPR-based reprogramming of gene expression will be presented. The review will focus on the designs implemented in yeast both at the level of CRISPR proteins and guide RNA (gRNA), but will lend due credits to the seminal studies performed in other species where relevant. In addition to design principles, this review also highlights applications benefitting from the use of CRISPR-mediated transcriptional regulation and discusses the future directions to further expand the toolkit for nuclease-deficient reprogramming of genomes. As such, this review should be of general interest for experimentalists to get familiarised with the parameters underlying the power of reprogramming genomic functions by use of nuclease-deficient CRISPR technologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.