Evidence map›Paper›PMID 29726060›Full record

ArticleStem cells (Dayton, Ohio)2018

Differential Regulation of Human Bone Marrow Mesenchymal Stromal Cell Chondrogenesis by Hypoxia Inducible Factor-1α Hydroxylase Inhibitors.

Dheraj K Taheem, Daniel A Foyt, Sandra Loaiza, Silvia A Ferreira, Dusko Ilic, Holger W Auner, Agamemnon E Grigoriadis, Gavin Jell, Eileen Gentleman

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it, 70 citations in OpenAlex.

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  17. Antioxidants (Basel, Switzerland) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Dheraj K TaheemCentre for Craniofacial and Regenerative Biology, Women's Health Academic Centre KHP, King's College London, London, United Kingdom.ORCID 0000-0002-0252-6677
Daniel A FoytCentre for Craniofacial and Regenerative Biology, Women's Health Academic Centre KHP, King's College London, London, United Kingdom.
Sandra LoaizaCancer Cell Protein Metabolism Group, Department of Medicine, Imperial College London, London, United Kingdom.
Silvia A FerreiraCentre for Craniofacial and Regenerative Biology, Women's Health Academic Centre KHP, King's College London, London, United Kingdom.
Dusko IlicDivision of Women's Health, Women's Health Academic Centre KHP, King's College London, London, United Kingdom.
Holger W AunerCancer Cell Protein Metabolism Group, Department of Medicine, Imperial College London, London, United Kingdom.
Agamemnon E GrigoriadisCentre for Craniofacial and Regenerative Biology, Women's Health Academic Centre KHP, King's College London, London, United Kingdom.
Gavin JellDivision of Surgery & Interventional Science, University College London, London, United Kingdom.
Eileen GentlemanCentre for Craniofacial and Regenerative Biology, Women's Health Academic Centre KHP, King's College London, London, United Kingdom.
King's College London · GBImperial College London · GBUniversity College London · GB

Funding

Cancer Research UK 15448Wellcome Trust
6 · The paper itself

Abstract

The transcriptional profile induced by hypoxia plays important roles in the chondrogenic differentiation of marrow stromal/stem cells (MSC) and is mediated by the hypoxia inducible factor (HIF) complex. However, various compounds can also stabilize HIF's oxygen-responsive element, HIF-1α, at normoxia and mimic many hypoxia-induced cellular responses. Such compounds may prove efficacious in cartilage tissue engineering, where microenvironmental cues may mediate functional tissue formation. Here, we investigated three HIF-stabilizing compounds, which each have distinct mechanisms of action, to understand how they differentially influenced the chondrogenesis of human bone marrow-derived MSC (hBM-MSC) in vitro. hBM-MSCs were chondrogenically-induced in transforming growth factor-β3-containing media in the presence of HIF-stabilizing compounds. HIF-1α stabilization was assessed by HIF-1α immunofluorescence staining, expression of HIF target and articular chondrocyte specific genes by quantitative polymerase chain reaction, and cartilage-like extracellular matrix production by immunofluorescence and histochemical staining. We demonstrate that all three compounds induced similar levels of HIF-1α nuclear localization. However, while the 2-oxoglutarate analog dimethyloxalylglycine (DMOG) promoted upregulation of a selection of HIF target genes, desferrioxamine (DFX) and cobalt chloride (CoCl

Indexed as

Amino Acids, DicarboxylicBone Marrow CellsCell DifferentiationCell HypoxiaChildChondrogenesisCobaltDeferoxamineEnzyme InhibitorsHumansHypoxia-Inducible Factor 1, alpha SubunitHypoxia-Inducible Factor-Proline DioxygenasesMesenchymal Stem CellsAmino Acids, DicarboxylicCobaltcobaltous chlorideDeferoxamineEnzyme InhibitorsHypoxia-Inducible Factor 1, alpha SubunitHypoxia-Inducible Factor-Proline DioxygenasesoxalylglycineBone marrow stromal cells (BMSCs)Cell signalingChondrogenesisDifferentiationHypoxiaMesenchymal stem cells (MSCs)Tissue regeneration

Identifiers

PMID29726060
PMCPMC6124654
OpenAlexW2800263359

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.