ArticleJournal of assisted reproduction and genetics2018
Continuous embryo culture elicits higher blastulation but similar cumulative delivery rates than sequential: a large prospective study.
Article in Journal of assisted reproduction and genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Do IVF culture conditions have an impact on neonatal outcomes? A systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2024Pooled it
- Another round, another chance: oocyte developmental competence and outcomes in second IVF attempts-the earlier, the better.Journal of assisted reproduction and genetics · 2026Observational
- A standardized training allows achieving similar clinical pregnancy rates per top-quality euploid blastocyst transfer across operators.Journal of assisted reproduction and genetics · 2025Article
- Embryo Culture Medium Has No Impact on Mosaicism Rates: a Sibling Oocyte Study.Reproductive sciences (Thousand Oaks, Calif.) · 2023Observational
- Metaphase-II oocyte competence is unlinked to the gonadotrophins used for ovarian stimulation: a matched case-control study in women of advanced maternal age.Journal of assisted reproduction and genetics · 2023Article
- The impact of single-step and sequential embryo culture systems on obstetric and perinatal outcomes in singleton pregnancies: the Massachusetts Outcomes Study of Assisted Reproductive Technology.Fertility and sterility · 2022Article
- Euploidy rates are not affected when embryos are cultured in a continuous (CCM) or sequential culture medium (SCM): a sibling oocyte study.Journal of assisted reproduction and genetics · 2021Observational
- Embryo blastulation and quality between days 5 and 6 of extended embryo culture.Journal of assisted reproduction and genetics · 2021Observational
- Oocyte competence is independent of the ovulation trigger adopted: a large observational study in a setting that entails vitrified-warmed single euploid blastocyst transfer.Journal of assisted reproduction and genetics · 2021Observational
- The usefulness of metaphase I oocytes in women who undergo controlled ovarian hyperstimulation for intracytoplasmic sperm injection.JBRA assisted reproduction · 2021Article
- Review
- A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos.Scientific reports · 2020Article
- Impact of Day 7 Blastocyst Transfer on Obstetric and Perinatal Outcome of Singletons Born After Vitrified-Warmed Embryo Transfer.Frontiers in physiology · 2020Article
- The impact of culture conditions on blastocyst formation and aneuploidy rates: a comparison between single-step and sequential media in a large academic practice.Journal of assisted reproduction and genetics · 2020Article
- Correlation analysis between C natriuretic peptide and pregnancy outcome.American journal of translational research · 2020Article
- Good practice recommendations for the use of time-lapse technologyHuman reproduction open · 2020Article
- The clinical use of time-lapse in human-assisted reproduction.Therapeutic advances in reproductive healthReview
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo assess whether continuous embryo culture involves better embryological and/or clinical outcomes than sequential.
methodsProspective study at a private IVF center. All consecutive IVF cycles (September 2013-2015) fulfilling the inclusion criteria underwent embryo culture in either Continuous-Single-Culture-Media (CSCM, n = 972) or sequential media (Quinn's Advantage, n = 514), respectively. ICSI, blastocyst culture in either standard (MINC) or undisturbed (Embryoscope) incubation, transfer (until September 2016), and pregnancy follow-up (until September 2017) were performed. When aneuploidy testing was required, trophectoderm biopsy and qPCR were performed. Sub-analyses and logistic regression corrected for confounders were performed. The primary outcomes were overall blastocyst rate per oocyte and mean blastocyst rate per cycle. The sample size was defined to reach 95 and 80% statistical power for the former and the latter outcome, respectively. Secondary outcomes were euploidy (if assessed), cumulative delivery rates, gestational age, and birthweight.
resultsContinuous embryo culture resulted into a higher overall blastocyst rate per inseminated oocyte than sequential (n = 2211/5841, 37.9% vs. 1073/3216, 33.4%; p < 0.01), confirmed also from a cycle-based analysis (mean blastocyst rate: 38.7% ± 29.7% vs. 34.3% ± 29.4%; p = 0.01). The continuous media (OR = 1.23), the undisturbed incubation system (OR = 1.22), the maternal age (OR = 0.92), and the sperm factor (OR = 0.85) were outlined as positive predictors of blastulation. However, the cumulative delivery rates per ended cycle (i.e., delivery achieved or no blastocyst produced or left; > 90%) were comparable in the two groups (n = 244/903, 27.0% vs. 129/475, 27.2%). The neonatal outcomes were similar.
conclusionsContinuous culture involves better embryological but similar clinical outcomes than sequential. This large prospective study supports the absence of clinical disparity among the two approaches.
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