ArticleScience advances2018
The role of electron irradiation history in liquid cell transmission electron microscopy.
Article in Science advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Imaging of Hydrated and Living Cells in Transmission Electron Microscope: Summary, Challenges, and Perspectives.ACS nano · 2025Review
- The Influence of Ionizing Radiation on Quantification for In Situ and Operando Liquid-Phase Electron Microscopy.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Exploring Biomineralization Processes Using In Situ Liquid Transmission Electron Microscopy: A Review.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Precision of Radiation Chemistry Networks: Playing Jenga with Kinetic Models for Liquid-Phase Electron Microscopy.Precision chemistry · 2023Article
- Assessing an aqueous flow cell designed for in situ crystal growth under X-ray nanotomography and effects of radiolysis products.Journal of synchrotron radiation · 2023Article
- Liquid-cell transmission electron microscopy for imaging of thermosensitive recombinant polymers.Journal of controlled release : official journal of the Controlled Release Society · 2022Article
- In Situ TEM Imaging of Solution-Phase Chemical Reactions Using 2D-Heterostructure Mixing Cells.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Article
- Self-assembling peptides imaged by correlated liquid cell transmission electron microscopy and MALDI-imaging mass spectrometry.Nature communications · 2019Article
- The role of nanoparticle size and ligand coverage in size focusing of colloidal metal nanoparticles.Nanoscale advances · 2019Article
- In Situ Identification of Nanoparticle Structural Information Using Optical Microscopy.The journal of physical chemistry letters · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In situ liquid cell transmission electron microscopy (LC-TEM) allows dynamic nanoscale characterization of systems in a hydrated state. Although powerful, this technique remains impaired by issues of repeatability that limit experimental fidelity and hinder the identification and control of some variables underlying observed dynamics. We detail new LC-TEM devices that improve experimental reproducibility by expanding available imaging area and providing a platform for investigating electron flux history on the sample. Irradiation history is an important factor influencing LC-TEM results that has, to this point, been largely qualitatively and not quantitatively described. We use these devices to highlight the role of cumulative electron flux history on samples from both nanoparticle growth and biological imaging experiments and demonstrate capture of time zero, low-dose images on beam-sensitive samples. In particular, the ability to capture pristine images of biological samples, where the acquired image is the first time that the cell experiences significant electron flux, allowed us to determine that nanoparticle movement compared to the cell membrane was a function of cell damage and therefore an artifact rather than visualizing cell dynamics in action. These results highlight just a subset of the new science that is accessible with LC-TEM through the new multiwindow devices with patterned focusing aides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.