Evidence map›Paper›PMID 29725363›Full record

ArticleExperimental and therapeutic medicine2018

Overexpression of Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains induces cluster of differentiation 59-mediated apoptosis in Jurkat cells.

Bei-Bei Cong, Mei-Hua Gao, Bing Li, Bing Wang, Bei Zhang, Li-Na Wang, Shu-Chao Zhang, Hua-Qiao Li, Zhong Wang, Shu-Yi Han

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental and therapeutic medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Regulation, targets and functions of CSK.Frontiers in cell and developmental biology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Bei-Bei CongDepartment of Immunology, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Mei-Hua GaoDepartment of Immunology, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Bing LiDepartment of Genetics, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Bing WangDepartment of Immunology, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Bei ZhangDepartment of Immunology, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Li-Na WangDepartment of Blood Transfusion, Shandong Qilu Hospital, Qingdao, Shandong 266071, P.R. China.
Shu-Chao ZhangDepartment of Blood Transfusion, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Hua-Qiao LiDepartment of Immunology, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Zhong WangDepartment of Immunology, Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China.
Shu-Yi HanMedical Research and Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250000, P.R. China.
Qingdao University · CNQilu Hospital of Shandong University · CNShandong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomains (CBP/PAG) is a membrane-bound adaptor protein that downregulates the activation of Src family kinases present in lipid rafts. To elucidate the role of CBP/PAG in human T cell activation, a cell line overexpressing CBP/PAG was constructed and the function of CBP/PAG in Jurkat cells was examined. The present study revealed that increased CBP/PAG expression in T cells significantly enhanced their apoptosis and reduced cellular activation and proliferation. Overexpression of CBP/PAG suppressed the growth of Jurkat cells by recruiting c-Src and its negative regulator, C-terminal Src kinase (CSK), to lipid rafts. The negative regulation of CBP/PAG was enhanced in the presence of anti-cluster of differentiation (CD)59 monoclonal antibodies. In addition, a significant association was revealed between the location of CBP/PAG and CD59, which were co-expressed in the same region of the cell membrane, implicating a potential overlap of the elicited signaling pathways. These results indicate that CBP/PAG functions as a negative regulator of cell signal transduction and suggest that CD59 may strengthen the role of negative feedback regulation.

Indexed as

apoptosiscluster of differentiation 59Csk-binding protein/phosphoprotein associated with glycosphingolipid-enriched microdomainsJurkat cellssignal transduction

Identifiers

PMID29725363
PMCPMC5920370
OpenAlexW2793873848

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.