ArticleFrontiers in molecular neuroscience2018
Fine Localization of Acetylcholinesterase in the Synaptic Cleft of the Vertebrate Neuromuscular Junction.
Article in Frontiers in molecular neuroscience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- New Insights into Neuromuscular Junction Biology: Evidence from Human and Animal Research.International journal of molecular sciences · 2026Review
- Unveiling the nanotoxicology of snake venoms through functional and biochemical characterization of extracellular vesicles from Naja naja and Daboia russelii.Scientific reports · 2025Article
- Distal electrical stimulation enhances neuromuscular reinnervation and satellite cell differentiation for functional recovery.Stem cell research & therapy · 2025Article
- Alkaloid Profile, Anticholinesterase and Antioxidant Activities, and Sexual Propagation inPlants (Basel, Switzerland) · 2025Article
- An α7 nicotinic and GABAThe Journal of physiology · 2025Article
- Astragaloside IV as a Memory-Enhancing Agent: In Silico Studies with In Vivo Analysis and Post Mortem ADME-Tox Profiling in Mice.International journal of molecular sciences · 2024Article
- Alzheimer's Disease: A Review of Pathology, Current Treatments, and the Potential Therapeutic Effect of Decreasing Oxidative Stress by Combined Vitamin D and l-Cysteine Supplementation.Antioxidants & redox signaling · 2024Review
- Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling.Nature neuroscience · 2024Article
- Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3.Science advances · 2023Article
- Post-synaptic specialization of the neuromuscular junction: junctional folds formation, function, and disorders.Cell & bioscience · 2022Review
- Review
- Acetylcholinesterase Inhibitors in the Treatment of Neurodegenerative Diseases and the Role of Acetylcholinesterase in their Pathogenesis.International journal of molecular sciences · 2021Review
- Resistance to Neuromuscular Blockade by Rocuronium in Surgical Patients with Spastic Cerebral Palsy.Journal of personalized medicine · 2021Article
- Anti-Cholinesterase Combination Drug Therapy as a Potential Treatment for Alzheimer's Disease.Brain sciences · 2021Article
- Cholinesterases in Tripartite Neuromuscular Synapse.Frontiers in molecular neuroscience · 2021Review
- Forebrain Cholinergic Signaling: Wired and Phasic, Not Tonic, and Causing Behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Review
- Diverse Spatiotemporal Scales of Cholinergic Signaling in the Neocortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Review
- Autoregulation of Acetylcholine Release and Micro-Pharmacodynamic Mechanisms at Neuromuscular Junction: Selective Acetylcholinesterase Inhibitors for Therapy of Myasthenic Syndromes.Frontiers in pharmacology · 2018Review
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetylcholinesterase (AChE) is concentrated at cholinergic synapses, where it is a major factor in controlling the duration of transmitter action. The concentration and localization of AChE within the synaptic cleft are in keeping with the functional requirements of the particular type of synapse. The densities of synaptic AChE at various neuromuscular junctions (NMJs) had been evaluated by quantitative EM-autoradiography using radiolabeled probes. Yet, fundamental issues concerning the precise distribution and location of the enzyme in the cleft remained open: whether and to what extent synaptic AChE is associated with pre- or postsynaptic membranes, or with synaptic basal lamina (BL), and whether it occurs only in the primary cleft (PC) or also in postjunctional folds (PJFs). Nanogold-conjugates of fasciculin, an anticholinesterase polypeptide toxin, were prepared and used to label AChE at NMJs of mouse and frog muscles. Selective intense labeling was obtained at the NMJs, with gold-labeled AChE sites distributed over the BL in the PC and the PJFs. Quantitative analysis demonstrated that AChE sites are almost exclusively located on the BL rather than on pre- or postsynaptic membranes and are distributed in the PC and down the PJFs, with a defined pattern. This localization pattern of AChE is suggested to ensure full hydrolysis of acetylcholine (ACh) bouncing off receptors, thus eliminating its unnecessary detrimental reattachment.
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