ArticleScientific reports2018
Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Mesenchymal Stem Cell-Derived Exosomes for Ovarian Reserve: A Systematic Review and Network Meta‑Analysis Based on Animal Studies.Stem cell reviews and reports · 2026Review
- Outside the box: comparative stem cell insights for fertility biotechnology and conservation.F&S reports · 2025Article
- Ovarian microenvironment: challenges and opportunities in protecting against chemotherapy-associated ovarian damage.Human reproduction update · 2024Review
- Oogonial stem cells: the unexpected superheroes.Reproduction & fertility · 2024Article
- Reconstruction of ovarian follicular-like structure by recellularization of a cell-free human ovarian scaffold with mouse fetal ovarian cells.Cytotechnology · 2024Article
- Mitochondria: the epigenetic regulators of ovarian aging and longevity.Frontiers in endocrinology · 2024Review
- Making a good egg: human oocyte health, aging, and in vitro development.Physiological reviews · 2023Review
- Review
- Metformin promotes female germline stem cell proliferation by upregulating Gata-binding protein 2 with histone β-hydroxybutyrylation.Stem cell research & therapy · 2023Article
- An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide.Turkish journal of obstetrics and gynecology · 2023Article
- The Continued Absence of Functional Germline Stem Cells in Adult Ovaries.Stem cells (Dayton, Ohio) · 2023Article
- Revisiting Claims of the Continued Absence of Functional Germline Stem Cells in Adult Ovaries.Stem cells (Dayton, Ohio) · 2023Article
- Characterization of Oogonial Stem Cells in Adult Mouse Ovaries with Age and Comparison to In Silico Data on Human Ovarian Aging.Stem cells and development · 2023Article
- Current progress onFrontiers in cell and developmental biology · 2023Review
- Female germline stem cells: aging and anti-aging.Journal of ovarian research · 2022Review
- Workflow Optimization for Identification of Female Germline or Oogonial Stem Cells in Human Ovarian Cortex Using Single-Cell RNA Sequence Analysis.Stem cells (Dayton, Ohio) · 2022Article
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Non-neutral clonal selection and its potential role in mammalian germline stem cell dysfunction with advancing age.Frontiers in cell and developmental biology · 2022Review
- Artificial Ovary for Young Female Breast Cancer Patients.Frontiers in medicine · 2022Review
- Germ cells of the mammalian female: A limited or renewable resource?†.Biology of reproduction · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The existence of a population of putative stem cells with germline developmental potential (oogonial stem cells: OSCs) in the adult mammalian ovary has been marked by controversy over isolation methodology and potential for in-vitro transformation, particularly where cell sorting has been based on expression of DEAD box polypeptide 4 (DDX4). This study describes a refined tissue dissociation/fluorescence-activated cell sorting (FACS) protocol for the ovaries of adult women which results in increased cell viability and yield of putative OSCs. A FACS technique incorporating dual-detection of DDX4 with aldehyde dehydrogenase 1 (ALDH1) demonstrates the existence of two sub-populations of small DDX4-positive cells (approx. 7 µm diameter) with ALDH1 activity, distinguished by expression of differentially spliced DDX4 transcripts and of DAZL, a major regulator of germ cell differentiation. These may indicate stages of differentiation from a progenitor population and provide a likely explanation for the expression disparities reported previously. These findings provide a robust basis for the further characterisation of these cells, and exploration of their potential physiological roles and therapeutic application.
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