ArticleMolecular microbiology2018
PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
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- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- Structural foundation for the role of enterococcal PrgB in conjugation, biofilm formation, and virulence.eLife · 2023Article
- Evaluation of Enterococcal Probiotic Usage and Review of Potential Health Benefits, Safety, and Risk of Antibiotic-Resistant Strain Emergence.Antibiotics (Basel, Switzerland) · 2023Review
- Domain shuffling of a highly mutable ligand-binding fold drives adhesin generation across the bacterial kingdom.Proteins · 2023Article
- Conjugation's Toolkit: the Roles of Nonstructural Proteins in Bacterial Sex.Journal of bacteriology · 2023Review
- The Regulations of Essential WalRK Two-Component System onJournal of clinical medicine · 2023Review
- Anti-Biofilm and Antibacterial Activities ofAntibiotics (Basel, Switzerland) · 2022Article
- Enterococcal biofilm-A nidus for antibiotic resistance transfer?Journal of applied microbiology · 2022Review
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- Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture.International journal of molecular sciences · 2021Review
- The HIV-1 nucleocapsid chaperone protein forms locally compacted globules on long double-stranded DNA.Nucleic acids research · 2021Article
- Article
- Understanding the Matrix: The Role of Extracellular DNA in Oral Biofilms.Frontiers in oral health · 2021Review
- Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis.Poultry science · 2020Article
- The Multifaceted Nature of Streptococcal Antigen I/II Proteins in Colonization and Disease Pathogenesis.Frontiers in microbiology · 2020Review
- Polymer Adhesin Domains in Gram-Positive Cell Surface Proteins.Frontiers in microbiology · 2020Review
- Biological and Structural Diversity of Type IV Secretion Systems.Microbiology spectrum · 2019Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Gram-positive bacteria deploy type IV secretion systems (T4SSs) to facilitate horizontal gene transfer. The T4SSs of Gram-positive bacteria rely on surface adhesins as opposed to conjugative pili to facilitate mating. Enterococcus faecalis PrgB is a surface adhesin that promotes mating pair formation and robust biofilm development in an extracellular DNA (eDNA) dependent manner. Here, we report the structure of the adhesin domain of PrgB. The adhesin domain binds and compacts DNA in vitro. In vivo PrgB deleted of its adhesin domain does not support cellular aggregation, biofilm development and conjugative DNA transfer. PrgB also binds lipoteichoic acid (LTA), which competes with DNA binding. We propose that PrgB binding and compaction of eDNA facilitates cell aggregation and plays an important role in establishment of early biofilms in mono- or polyspecies settings. Within these biofilms, PrgB mediates formation and stabilization of direct cell-cell contacts through alternative binding of cell-bound LTA, which in turn promotes establishment of productive mating junctions and efficient intra- or inter-species T4SS-mediated gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.