ArticleJournal of experimental botany2018
A missense allele of KARRIKIN-INSENSITIVE2 impairs ligand-binding and downstream signaling in Arabidopsis thaliana.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 37 citations in OpenAlex.
- Negative feedback regulation of karrikin signaling inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Molecular insights into volatile organic compound sensing and signaling in plants.The Plant journal : for cell and molecular biology · 2026Review
- The Multifaceted Impact of Karrikin Signaling in Plants.International journal of molecular sciences · 2025Review
- Identification and structure-guided development of triazole urea-based selective antagonists of Arabidopsis karrikin signaling.Nature communications · 2025Article
- HTL/KAI2 signaling substitutes for light to control plant germination.PLoS genetics · 2024Article
- A KARRIKIN INSENSITIVE2 paralog in lettuce mediates highly sensitive germination responses to karrikinolide.Plant physiology · 2022Article
- SMAX1 potentiates phytochrome B-mediated hypocotyl thermomorphogenesis.The Plant cell · 2022Article
- KARRIKIN UP-REGULATED F-BOX 1 (KUF1) imposes negative feedback regulation of karrikin and KAI2 ligand metabolism inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural and functional analyses explain Pea KAI2 receptor diversity and reveal stereoselective catalysis during signal perception.Communications biology · 2022Article
- The mechanism of host-induced germination in root parasitic plants.Plant physiology · 2021Review
- A Raf-like kinase is required for smoke-induced seed dormancy inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structure-Function Analysis of SMAX1 Reveals Domains That Mediate Its Karrikin-Induced Proteolysis and Interaction with the Receptor KAI2.The Plant cell · 2020Article
- Perception of karrikins by plants: a continuing enigma.Journal of experimental botany · 2020Review
- Divergent receptor proteins confer responses to different karrikins in two ephemeral weeds.Nature communications · 2020Article
- Potential of Karrikins as Novel Plant Growth Regulators in Agriculture.Plants (Basel, Switzerland) · 2019Review
- Impairment in karrikin but not strigolactone sensing enhances root skewing in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2019Article
- KAI2-KL signaling intersects with light-signaling for photomorphogenesis.Plant signaling & behavior · 2019Article
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19 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A smoke-derived compound, karrikin (KAR), and an endogenous but as yet unidentified KARRIKIN INSENSITIVE2 (KAI2) ligand (KL) have been identified as chemical cues in higher plants that impact on multiple aspects of growth and development. Genetic screening of light-signaling mutants in Arabidopsis thaliana has identified a mutant designated as ply2 (pleiotropic long hypocotyl2) that has pleiotropic light-response defects. In this study, we used positional cloning to identify the molecular lesion of ply2 as a missense mutation of KAI2/HYPOSENSITIVE TO LIGHT, which causes a single amino acid substitution, Ala219Val. Physiological analysis and genetic epistasis analysis with the KL-signaling components MORE AXILLARY GROWTH2 (MAX2) and SUPPRESSOR OF MAX2 1 suggested that the pleiotropic phenotypes of the ply2 mutant can be ascribed to a defect in KL-signaling. Molecular and biochemical analyses revealed that the mutant KAI2ply2 protein is impaired in its ligand-binding activity. In support of this conclusion, X-ray crystallography studies suggested that the KAI2ply2 mutation not only results in a narrowed entrance gate for the ligand but also alters the structural flexibility of the helical lid domains. We discuss the structural implications of the Ala219 residue with regard to ligand-specific binding and signaling of KAI2, together with potential functions of KL-signaling in the context of the light-regulatory network in Arabidopsis thaliana.
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