ArticleAmerican journal of physiology. Lung cellular and molecular physiology2018
Long-term culture and cloning of primary human bronchial basal cells that maintain multipotent differentiation capacity and CFTR channel function.
Article in American journal of physiology. Lung cellular and molecular physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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Who cites it
39 citing papers in PubMed.
- Article
- A serum-free culture medium for long-term expansion of human airway basal stem cells.Stem cell research & therapy · 2026Article
- Deciphering the role of the Sch9 serine/threonine kinase inFrontiers in fungal biology · 2026Article
- Isolation and long-term culture of primary mouse cholangiocytes that retain biophysical properties and distinct Cl-conductances: An initial study.Physiological reports · 2026Article
- Review
- Culture conditions differentially regulate the inflammatory niche and cellular phenotype of tracheobronchial basal stem cells.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Defective Cystic Fibrosis Transmembrane Conductance Regulator Accelerates Skeletal Muscle Aging by Impairing Autophagy/Myogenesis.Journal of cachexia, sarcopenia and muscle · 2025Article
- Culture Conditions Differentially Regulate the Inflammatory Niche and Cellular Phenotype of Tracheo-Bronchial Basal Stem Cells.bioRxiv : the preprint server for biology · 2024Article
- Differentiation of CD166-positive hPSC-derived lung progenitors into airway epithelial cells.Biology open · 2024Article
- Breathing on chip: Dynamic flow and stretch accelerate mucociliary maturation of airway epitheliumMaterials today. Bio · 2023Article
- Molecular and Functional Characteristics of Airway Epithelium under Chronic Hypoxia.International journal of molecular sciences · 2023Article
- Conditionally reprogrammed asthmatic bronchial epithelial cells express lowerAmerican journal of physiology. Lung cellular and molecular physiology · 2022Article
- Serially passaged, conditionally reprogrammed nasal epithelial cells as a model to study epithelial functions and SARS-CoV-2 infection.American journal of physiology. Cell physiology · 2022Article
- DNA damage response at telomeres boosts the transcription of SARS-CoV-2 receptor ACE2 during aging.EMBO reports · 2022Article
- Higher throughput drug screening for rare respiratory diseases: readthrough therapy in primary ciliary dyskinesia.The European respiratory journal · 2021Article
- National Heart, Lung, and Blood Institute and Building Respiratory Epithelium and Tissue for Health (BREATH) Consortium Workshop Report: Moving Forward in Lung Regeneration.American journal of respiratory cell and molecular biology · 2021Article
- Stem Cell-derived Respiratory Epithelial Cell Cultures as Human Disease Models.American journal of respiratory cell and molecular biology · 2021Review
- Long-term differentiating primary human airway epithelial cell cultures: how far are we?Cell communication and signaling : CCS · 2021Review
- Rho/SMAD/mTOR triple inhibition enables long-term expansion of human neonatal tracheal aspirate-derived airway basal cell-like cells.Pediatric research · 2021Article
- Implications for Extracellular Matrix Interactions With Human Lung Basal Stem Cells in Lung Development, Disease, and Airway Modeling.Frontiers in pharmacology · 2021Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
While primary cystic fibrosis (CF) and non-CF human bronchial epithelial basal cells (HBECs) accurately represent in vivo phenotypes, one barrier to their wider use has been a limited ability to clone and expand cells in sufficient numbers to produce rare genotypes using genome-editing tools. Recently, conditional reprogramming of cells (CRC) with a Rho-associated protein kinase (ROCK) inhibitor and culture on an irradiated fibroblast feeder layer resulted in extension of the life span of HBECs, but differentiation capacity and CF transmembrane conductance regulator (CFTR) function decreased as a function of passage. This report details modifications to the standard HBEC CRC protocol (Mod CRC), including the use of bronchial epithelial cell growth medium, instead of F medium, and 2% O
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