ArticleJournal of microbiology (Seoul, Korea)2018
An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus.
Article in Journal of microbiology (Seoul, Korea), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
- GSNOR is essential for nitric oxide homeostasis and involved in aflatoxin biosynthesis and pathogenicity inApplied and environmental microbiology · 2026Article
- A PEG-mediated protoplast transformation system for EGFP labeling of the shot-hole pathogen Wilsonomyces carpophilus.Frontiers in microbiology · 2026Article
- Development of Green Fluorescent Protein-Tagged Strains ofMicroorganisms · 2024Article
- Establishment of anCurrent issues in molecular biology · 2024Article
- Role of Flavohemoglobins in the Development and Aflatoxin Biosynthesis ofJournal of fungi (Basel, Switzerland) · 2024Article
- Synthetic Biology Tools for EngineeringJournal of fungi (Basel, Switzerland) · 2024Review
- Homologous Expression and Characterization of α-L-rhamnosidase from Aspergillus niger for the Transformation of Flavonoids.Applied biochemistry and biotechnology · 2022Article
- Systematic Characterization of bZIP Transcription Factors Required for Development and Aflatoxin Generation by High-Throughput Gene Knockout inJournal of fungi (Basel, Switzerland) · 2022Article
- Genetic Manipulation and Transformation Methods forMycobiology · 2021Review
- Article
- Aromatic Polyketides from a Symbiotic StrainMarine drugs · 2020Article
- Genome-wide association study leads to novel genetic insights into resistance to Aspergillus flavus in maize kernels.BMC plant biology · 2020Article
- A Novel Site-Specific Integration System for Genetic Modification ofG3 (Bethesda, Md.) · 2020Article
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in this fungus has not been well explored mainly due to the lack of an efficient transformation method for constructing a genome-wide gene mutant library. This challenge was resolved in this study, where a reliable and efficient Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for A. flavus NRRL 3357 was established. The results showed that removal of multinucleate conidia, to collect a homogenous sample of uninucleate conidia for use as the transformation material, is the key step in this procedure. A. tumefaciens strain AGL-1 harboring the ble gene for zeocin resistance under the control of the gpdA promoter from A. nidulans is suitable for genetic transformation of this fungus. We successfully generated A. flavus transformants with an efficiency of ∼ 60 positive transformants per 10
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