ArticleStem cell research & therapy2018
Efficient induction of functional ameloblasts from human keratinocyte stem cells.
Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Next-Generation Strategies for Enamel Repair and Regeneration: Advances in Biomaterials and Translational Challenges.Tissue engineering and regenerative medicine · 2025Review
- Subcutaneous implantation of tooth germ stem cells over the masseter muscle in mice: An in vivo pilot study.Regenerative therapy · 2025Article
- KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF-κB axis.Journal of cellular physiology · 2024Article
- Unraveling the hidden complexity: Exploring dental tissues through single-cell transcriptional profiling.Regenerative therapy · 2024Review
- Induction of human stem cells into ameloblasts by reaggregation strategy.Stem cell research & therapy · 2024Article
- Hard Dental Tissues Regeneration-Approaches and Challenges.Materials (Basel, Switzerland) · 2021Review
- Cancer Stemness-Based Prognostic Immune-Related Gene Signatures in Lung Adenocarcinoma and Lung Squamous Cell Carcinoma.Frontiers in endocrinology · 2021Article
- Tooth Formation: Are the Hardest Tissues of Human Body Hard to Regenerate?International journal of molecular sciences · 2020Review
- Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update.Stem cells international · 2020Review
- Low temperature culture enhances ameloblastic differentiation of human keratinocyte stem cells.Journal of molecular histology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundAlthough adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the regenerated enamel retains a running pattern of prism identical to and acquires mechanical properties comparable with human enamel indeed warrants further study.
methodsCultured human keratinocyte stem cells (hKSCs) were treated with fibroblast growth factor 8 (FGF8) and Sonic hedgehog (SHH) for 18 h or 36 h prior to being recombined with E13.5 mouse dental mesenchyme with implantation of FGF8 and SHH-soaked agarose beads into reconstructed chimeric tooth germs. Recombinant tooth germs were subjected to kidney capsule culture in nude mice. Harvested samples at various time points were processed for histological, immunohistochemical, TUNEL, and western blot analysis. Scanning electronic microscopy and a nanoindentation test were further employed to analyze the prism running pattern and mechanical properties of the regenerated enamel.
resultsTreatment of hKSCs with both FGF8 and SHH prior to tissue recombination greatly enhanced the rate of tooth-like structure formation to about 70%. FGF8 and SHH dramatically enhanced stemness of cultured hKSCs. Scanning electron microscopic analysis revealed the running pattern of intact prisms of regenerated enamel is similar to that of human enamel. The nanoindentation test indicated that, although much softer than human child and adult mouse enamel, mechanical properties of the regenerated enamel improved as the culture time was extended.
conclusionsApplication of FGF8 and SHH proteins in cultured hKSCs improves stemness but does not facilitate odontogenic fate of hKSCs, resulting in an enhanced efficiency of ameloblastic differentiation of hKSCs and tooth formation in human-mouse chimeric tooth germs.
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