Evidence map›Paper›PMID 29720171›Full record

ArticleMicrobial cell factories2018

Improved ethanol productivity from lignocellulosic hydrolysates by Escherichia coli with regulated glucose utilization.

Jinfeng Sun, Kangming Tian, Jie Wang, Zixing Dong, Xiaoguang Liu, Kugenthiren Permaul, Suren Singh, Bernard A Prior, Zhengxiang Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Synthetic Biology and Metabolic Engineering EmployingFrontiers in bioengineering and biotechnology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Jinfeng SunCenter for Bioresource and Bioenergy, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
Kangming TianDepartment of Biological Chemical Engineering, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China.
Jie WangDepartment of Biological Chemical Engineering, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China.
Zixing DongDepartment of Biological Chemical Engineering, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China.
Xiaoguang LiuDepartment of Biological Chemical Engineering, College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China.
Kugenthiren PermaulDepartment of Biotechnology and Food Technology, Durban University of Technology, Durban, 4002, South Africa.
Suren SinghDepartment of Biotechnology and Food Technology, Durban University of Technology, Durban, 4002, South Africa.
Bernard A PriorDepartment of Microbiology, University of Stellenbosch, Matieland, 7602, South Africa.
Zhengxiang WangCenter for Bioresource and Bioenergy, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China. zxwang0519@tust.edu.cn.
Tianjin University of Science and Technology · CNDurban University of Technology · ZAHuaiyin Institute of Technology · CNStellenbosch University · ZA

Funding

The Raising Program of Innovation Team for Tianjin Universities TD12-5002
6 · The paper itself

Abstract

backgroundLignocellulosic ethanol could offer a sustainable source to meet the increasing worldwide demand for fuel. However, efficient and simultaneous metabolism of all types of sugars in lignocellulosic hydrolysates by ethanol-producing strains is still a challenge.

resultsAn engineered strain Escherichia coli B0013-2021HPA with regulated glucose utilization, which could use all monosaccharides in lignocellulosic hydrolysates except glucose for cell growth and glucose for ethanol production, was constructed. In E. coli B0013-2021HPA, pta-ackA, ldhA and pflB were deleted to block the formation of acetate, lactate and formate and additional three mutations at glk, ptsG and manZ generated to block the glucose uptake and catabolism, followed by the replacement of the wild-type frdA locus with the ptsG expression cassette under the control of the temperature-inducible λ pR and pL promoters, and the final introduction of pEtac-PA carrying Zymomonas mobilis pdc and adhB for the ethanol pathway. B0013-2021HPA was able to utilize almost all xylose, galactose and arabinose but not glucose for cell propagation at 34 °C and converted all sugars to ethanol at 42 °C under oxygen-limited fermentation conditions.

conclusionsEngineered E. coli strain with regulated glucose utilization showed efficient metabolism of mixed sugars in lignocellulosic hydrolysates and thus higher productivity of ethanol production.

Indexed as

Escherichia coliEthanolGlucoseLigninEthanolGlucoseLigninlignocelluloseEscherichia coliEthanolLignocellulosic hydrolysateThermo-regulated glucose utilization

Identifiers

PMID29720171
PMCPMC5930954
OpenAlexW2799921050

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.