ArticleNucleic acids research2018
T7 RNA polymerase non-specifically transcribes and induces disassembly of DNA nanostructures.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Self-assembled cell-scale containers made from DNA origami membranes.Nature materials · 2026Article
- Dynamic Control of DNA Origami Self-Assembly by Transcriptional Modules.Journal of the American Chemical Society · 2026Article
- Template nicking suppresses promoter-independent antisense transcription in IVT via R-loop-mediated strand displacement.Nucleic acids research · 2026Article
- Diversifying Substrates and Reaction Conditions for Polymerase Strand Recycling.ACS synthetic biology · 2025Article
- A cell-free biosensor signal amplification circuit with polymerase strand recycling.Nature chemical biology · 2025Article
- Spatial Control over Reactions via Localized Transcription within Membraneless DNA Nanostar Droplets.Journal of the American Chemical Society · 2024Article
- Directing Nanoparticle Organization in Response to Diverse Chemical Inputs.Journal of the American Chemical Society · 2024Article
- Strategies to Reduce Promoter-Independent Transcription of DNA Nanostructures and Strand Displacement Complexes.ACS synthetic biology · 2024Review
- Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems.Nature communications · 2024Article
- Functionalizing DNA Origami by Triplex-Directed Site-Specific Photo-Cross-Linking.Journal of the American Chemical Society · 2024Article
- Engineering a cell-free biosensor signal amplification circuit with polymerase strand recycling.bioRxiv : the preprint server for biology · 2024Article
- Nucleic acid nanostructures forApplied physics reviews · 2023Review
- Programming cell-free biosensors with DNA strand displacement circuits.Nature chemical biology · 2022Article
- Logic invades cell-free biosensing.Nature chemical biology · 2022Article
- Growth and site-specific organization of micron-scale biomolecular devices on living mammalian cells.Nature communications · 2021Article
- Dynamic self-assembly of compartmentalized DNA nanotubes.Nature communications · 2021Article
- Autonomous dynamic control of DNA nanostructure self-assembly.Nature chemistry · 2019Article
- Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems.Methods and protocols · 2019Review
- Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis.Nucleic acids research · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of proteins that bind and catalyze reactions with DNA alongside DNA nanostructures has broadened the functionality of DNA devices. DNA binding proteins have been used to specifically pattern and tune structural properties of DNA nanostructures and polymerases have been employed to directly and indirectly drive structural changes in DNA structures and devices. Despite these advances, undesired and poorly understood interactions between DNA nanostructures and proteins that bind DNA continue to negatively affect the performance and stability of DNA devices used in conjunction with enzymes. A better understanding of these undesired interactions will enable the construction of robust DNA nanostructure-enzyme hybrid systems. Here, we investigate the undesired disassembly of DNA nanotubes in the presence of viral RNA polymerases (RNAPs) under conditions used for in vitro transcription. We show that nanotubes and individual nanotube monomers (tiles) are non-specifically transcribed by T7 RNAP, and that RNA transcripts produced during non-specific transcription disassemble the nanotubes. Disassembly requires a single-stranded overhang on the nanotube tiles where transcripts can bind and initiate disassembly through strand displacement, suggesting that single-stranded domains on other DNA nanostructures could cause unexpected interactions in the presence of viral RNA polymerases.
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