Evidence map›Paper›PMID 29717998›Full record

ArticleActa crystallographica. Section F, Structural biology communications2018

In situ proteolysis of an N-terminal His tag with thrombin improves the diffraction quality of human aldo-keto reductase 1C3 crystals.

Jovana J Plavša, Pavlína Řezáčová, Michael Kugler, Petr Pachl, Jiří Brynda, Zdeněk Voburka, Anđelka Ćelić, Edward T Petri, Jana Škerlová

Abstract read
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In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. The structural basis of aldo-keto reductase 1C3 inhibition by 17α-picolyl and 17(Journal of enzyme inhibition and medicinal chemistry · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jovana J PlavšaDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia.
Pavlína ŘezáčováInstitute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
Michael KuglerInstitute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
Petr PachlInstitute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
Jiří BryndaInstitute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
Zdeněk VoburkaInstitute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
Anđelka ĆelićDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia.
Edward T PetriDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia.
Jana ŠkerlováInstitute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Flemingovo nám. 2, 166 10 Prague, Czech Republic.

Funding

Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja 172021Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja 173014Ministerstvo Školství, Mládeǽe a Tělovýchovy 8X17050Ministerstvo Školství, Mládeǽe a Tělovýchovy DS-2016-0050Ministerstvo Školství, Mládeǽe a Tělovýchovy NPU I: LO1304
6 · The paper itself

Abstract

Human aldo-keto reductase 1C3 (AKR1C3) stereospecifically reduces steroids and prostaglandins and is involved in the biotransformation of xenobiotics. Its role in various cancers makes it a potential therapeutic target for the development of inhibitors. Recombinant AKR1C3 with a thrombin-cleavable N-terminal His

Indexed as

HistidineAldo-Keto Reductase Family 1 Member C3Amino Acid SequenceCrystallizationCrystallography, X-RayHumansProteolysisThrombinX-Ray DiffractionAKR1C3 protein, humanAldo-Keto Reductase Family 1 Member C3HistidineThrombin17β-hydroxysteroid dehydrogenase 5aldo-keto reductase 1C3diffraction-quality improvementHis tagsin situ proteolysispET-28(+)

Identifiers

PMID29717998
PMCPMC5931143

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.