Evidence map›Paper›PMID 29717823›Full record

ArticleAdvanced healthcare materials2018

Thermoreversible Hyaluronic Acid-PNIPAAm Hydrogel Systems for 3D Stem Cell Culture.

Barbara L Ekerdt, Christina M Fuentes, Yuguo Lei, Maroof M Adil, Anusuya Ramasubramanian, Rachel A Segalman, David V Schaffer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed.

  1. Discrete 2D Material Programming for 3D Shaping and Morphogenesis-Inspired 4D Bioprinting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Recent Developments in Nanoparticle-Hydrogel Hybrid Materials for Controlled Release.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  7. Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing.Small (Weinheim an der Bergstrasse, Germany) · 2024
    Article
  13. Article
  14. Review
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Barbara L EkerdtDepartment of Chemical and Biolomolecular Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Christina M FuentesDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Yuguo LeiDepartment of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Lincoln, NE, 68588, USA.
Maroof M AdilDepartment of Chemical and Biolomolecular Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Anusuya RamasubramanianDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Rachel A SegalmanDepartment of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, 93106, USA.
David V SchafferDepartment of Chemical and Biolomolecular Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.

Funding

Mechanisms of Neural Stem Cell MechanoregulationR01NS074831 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Sanjay Kumar, DAVID V SCHAFFER · 2012 to 2026
$5.9M
Stem Cell Engineering Training ProgramT32GM098218 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD, SCHAFFER, DAVID V · 2011 to 2020
$1.8M
Multivalent Ligands to Control Stem Cell FateR01NS083856 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI KANE, RAVI S., SCHAFFER, DAVID V · 2014 to 2018
$1.7M
NIGMS NIH HHS T32 GM098218NINDS NIH HHS R01 NS074831NINDS NIH HHS R01 NS083856
6 · The paper itself

Abstract

Human pluripotent stem cells (hPSCs) offer considerable potential for biomedical applications including drug screening and cell replacement therapies. Clinical translation of hPSCs requires large quantities of high quality cells, so scalable methods for cell culture are needed. However, current methods are limited by scalability, the use of animal-derived components, and/or low expansion rates. A thermoresponsive 3D hydrogel for scalable hPSC expansion and differentiation into several defined lineages is recently reported. This system would benefit from increased control over material properties to further tune hPSC behavior, and here a scalable 3D biomaterial with the capacity to tune both the chemical and the mechanical properties is demonstrated to promote hPSC expansion under defined conditions. This 3D biomaterial, comprised of hyaluronic acid and poly(N-isopropolyacrylamide), has thermoresponsive properties that readily enable mixing with cells at low temperatures, physical encapsulation within the hydrogel upon elevation at 37 °C, and cell recovery upon cooling and reliquefaction. After optimization, the resulting biomaterial supports hPSC expansion over long cell culture periods while maintaining cell pluripotency. The capacity to modulate the mechanical and chemical properties of the hydrogel provides a new avenue to expand hPSCs for future therapeutic application.

Indexed as

Acrylic ResinsHyaluronic AcidHydrogelsCell Culture TechniquesCell LineHot TemperatureHumansPluripotent Stem CellsAcrylic ResinsHyaluronic AcidHydrogelspoly-N-isopropylacrylamidehyaluronic acidpoly(N-isopropylacrylamide)stem cell culturesthermoresponsive hydrogels

Identifiers

PMID29717823
PMCPMC6289514

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.