ArticleSheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi2017
[Visualized detection for mycobacterium tuberculosis using loop-mediated isothermal amplification assay].
Article in Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
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Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Low-complexity manual nucleic acid amplification tests for pulmonary tuberculosis in children.The Cochrane database of systematic reviews · 2025Pooled it
- Multiplex detection of blood-borne pathogens on a self-driven microfluidic chip using loop-mediated isothermal amplification.Analytical and bioanalytical chemistry · 2021Article
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3 authors.
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Abstract
In this study, loop-mediated isothermal amplification(LAMP) assay in conjunction with calcein for visualized detection of Mycobacterium tuberculosis(MTB) was established. Firstly, four LAMP primers were designed according to the region of 16 S r DNA sequences of MTB. Secondly, clinical sputum samples were collected,decontaminated and their DNA was extracted. Thirdly, standard MTB strains were used to evaluate the specificity and sensitivity of LAMP. At the same time, electrophoresis was used for products detection and calcein was used for visualized verification. At last, Chi-squared test function in SPSS 17.0 software was used for consistency evaluation of LAMP assay as compared with the gold standard(culture method). Results showed that there was no nonspecific amplification appeared in the specificity assay and the detection limit was 10 copies/tube in the sensitivity assay. In addition, visualized method by calcein had a comparable sensitivity with that of electrophoresis method. After evaluation of clinical practicability, the sensitivity of LAMP was calculated as 94.74% and the specificity was 90%, respectively. And Chi-squared test showed that LAMP and culture method had no statistic difference, and the two methods were in good consistency(P>0.05). In conclusion, LAMP assay introduced in our study has the characteristics of high efficiency and visualized detection so that this technique has great application prospects in the resource-limited environment, such as work field and primary care hospitals.
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29717591PMC9935362What OpenQuestion holds
Registered trials
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