Dechlorane Plus increases adipogenesis in 3T3-L1 and human primary preadipocytes independent of peroxisome proliferator-activated receptor γ transcriptional activity.

Vian Peshdary, Gabriella Calzadilla, Anne Landry, Alexander Sorisky, Ella Atlas

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Article in International journal of obesity (2005), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

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4citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 citing papers in PubMed.

Addressing chemically-induced obesogenic metabolic disruption: selection of chemicals forFrontiers in endocrinology · 2024
Review
An insight into brown/beige adipose tissue whitening, a metabolic complication of obesity with the multifactorial origin.Frontiers in endocrinology · 2023
Review
Generation of Functional Immortalized Human Corneal Stromal Stem Cells.International journal of molecular sciences · 2022
Article
Adipose Tissue and Endocrine-Disrupting Chemicals: Does Sex Matter?International journal of environmental research and public health · 2020
Review

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5 · Who and what money

Authors and funding

5 authors.

Vian PeshdaryEnvironmental Health Science and Research Bureau, Health Canada, 50 Colombine Driveway, Ottawa, ON, Canada.

Gabriella CalzadillaEnvironmental Health Science and Research Bureau, Health Canada, 50 Colombine Driveway, Ottawa, ON, Canada.

Anne LandryChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.

Alexander SoriskyOttawa Hospital Research Institute and Departments of Medicine and of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON, Canada.ORCID http://orcid.org/0000-0003-3507-0458

Ella AtlasEnvironmental Health Science and Research Bureau, Health Canada, 50 Colombine Driveway, Ottawa, ON, Canada. ella.atlas@canada.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

objectivesPolybrominated diphenyl ethers (PBDEs) are chemicals that were added to consumer products to reduce flammability but were deemed toxic and bioaccumulative and were phased out of commerce. Flame retardants (FRs) such as Dechlorane Plus (DP) were introduced as replacements. DP is being produced in high volumes and is detected in the environment, human milk, and human serum. Although human exposure to DP is evident, little is known about its potential effects on human health. We and others have shown that some FRs are potential obesogens, i.e., promote adipogenesis. However, the effects of DP on adipogenesis are not known.

methodsMurine 3T3-L1 and human primary subcutaneous (Sc) and omental (Om) preadipocytes were differentiated in the presence of DP (0.001-10 µM) and adipogenic effects were measured. Further, the ability of DP to activate the adipogenic transcription factor peroxisome proliferator-activated receptor γ (PPARγ) was also assessed.

resultsWe show that treatment of murine preadipocytes with DP significantly (p < 0.05) increased lipid accumulation (2.5-fold) and the mRNA expression of adipogenic markers: fatty acid binding protein 4 (Fabp4), lipoprotein lipase (Lpl), perilipin (Plin), adipsin, and adiponectin. DP also significantly (p < 0.05) increased the protein levels of selected mature adipocyte markers. We further show using luciferase reporter assays that DP increased PPARγ transcriptional activity by threefold (p < 0.05). When the PPARγ agonist was replaced by DP in the human preadipocyte differentiation cocktail, DP significantly (p < 0.05) increased the mRNA levels of adipogenic markers, PPARγ, FABP4, and PLIN in human Sc as well as Om cultures. Finally, PPARγ antagonist studies revealed that DP-mediated upregulation of adipogenic markers Fabp4 and Lpl did not occur via PPARγ activation.

conclusionThe current study shows that DP can induce adipogenesis of murine and human preadipocytes. We show that, although DP can directly activate PPARγ, its adipogenic effects may be mediated via other pathways.

Indexed as

3T3-L1 CellsAdipocytesAdipogenesisAdultAgedAnimalsCells, CulturedFemaleHumansHydrocarbons, ChlorinatedLipid MetabolismLipidsMaleMiceMiddle AgedPolycyclic Compoundsdechlorane plusHydrocarbons, ChlorinatedLipidsPolycyclic CompoundsPPAR gamma

Identifiers

PMID29717277

DOI10.1038/s41366-018-0072-7

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.