ArticleScientific reports2018
Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
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- Advancing cancer research through 3D cell culture models.EXCLI journal · 2025Review
- Assessment of cellular senescence potential of PM2.5 using 3D human lung fibroblast spheroids in vitro model.Toxicology research · 2024Article
- Biological characterization of breast cancer spheroid formed by fast fabrication method.In vitro models · 2024Article
- Robust formation of optimal single spheroids towards cost-effective in vitro three-dimensional tumor models.FEBS open bio · 2023Article
- Microfluidic Droplet-Assisted Fabrication of Vessel-Supported Tumors for Preclinical Drug Discovery.ACS applied materials & interfaces · 2023Article
- Hierarchical Vessel Network-Supported Tumor Model-on-a-Chip Constructed by Induced Spontaneous Anastomosis.ACS applied materials & interfaces · 2023Article
- Spheroid Engineering in Microfluidic Devices.ACS omega · 2023Review
- Article
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- Mammalian Cell Spheroids on Mixed Organic-Inorganic Superhydrophobic Coating.Molecules (Basel, Switzerland) · 2022Article
- Flipped Well-Plate Hanging-Drop Technique for Growing Three-Dimensional Tumors.Frontiers in bioengineering and biotechnology · 2022Article
- From cell spheroids to vascularized cancer organoids: Microfluidic tumor-on-a-chip models for preclinical drug evaluations.Biomicrofluidics · 2021Review
- Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis.Essays in biochemistry · 2021Review
- Synthesis of an AIEgen functionalized cucurbit[7]uril for subcellular bioimaging and synergistic photodynamic therapy and supramolecular chemotherapy.Chemical science · 2021Article
- Challenges of applying multicellular tumor spheroids in preclinical phase.Cancer cell international · 2021Review
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Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
A facile method for generation of tumor spheroids in large quantity with controllable size and high uniformity is presented. HCT-116 cells are used as a model cell line. Individual tumor cells are sparsely seeded onto petri-dishes. After a few days of growth, separated cellular islets are formed and then detached by dispase while maintaining their sheet shape. These detached cell sheets are transferred to dispase-doped media under orbital shaking conditions. Assisted by the shear flow under shaking and inhibition of cell-to-extracellular matrix junctions by dispase, the cell sheets curl up and eventually tumor spheroids are formed. The average size of the spheroids can be controlled by tuning the cell sheet culturing period and spheroid shaking period. The uniformity can be controlled by a set of sieves which were home-made using stainless steel meshes. Since this method is based on simple petri-dish cell culturing and shaking, it is rather facile for forming tumor spheroids with no theoretical quantity limit. This method has been used to form HeLa, A431 and U87 MG tumor spheroids and application of the formed tumor spheroids in drug screening is also demonstrated. The viability, 3D structure, and necrosis of the spheroids are characterized.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.