Evidence map›Paper›PMID 29717171›Full record

ArticleScientific reports2018

Elevated expression of human bHLH factor ATOH7 accelerates cell cycle progression of progenitors and enhances production of avian retinal ganglion cells.

Xiang-Mei Zhang, Takao Hashimoto, Ronald Tang, Xian-Jie Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Neurog2 regulates Isl1 to modulate horizontal cell number.Development (Cambridge, England) · 2023
    Article
  7. Genetic control of retinal ganglion cell genesis.Cellular and molecular life sciences : CMLS · 2021
    Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. On the Generation and Regeneration of Retinal Ganglion Cells.Frontiers in cell and developmental biology · 2020
    Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Xiang-Mei ZhangStein Eye Institute, University of California, Los Angeles, CA, USA.
Takao HashimotoStein Eye Institute, University of California, Los Angeles, CA, USA.
Ronald TangStein Eye Institute, University of California, Los Angeles, CA, USA.
Xian-Jie YangStein Eye Institute, University of California, Los Angeles, CA, USA. yang@jsei.ucla.edu.
University of California, Los Angeles · US

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8M
Neuroprotection Mechanism for PhotoreceptorsR01EY026319 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YANG, XIAN-JIE · 2016 to 2025
$4.1M
Hedgehog Signaling in Photoreceptor Differentiation and MaintenanceR01EY019052 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YANG, XIAN-JIE · 2010 to 2013
$1.5M
NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY019052NEI NIH HHS R01 EY026319
6 · The paper itself

Abstract

The production of vertebrate retinal projection neurons, retinal ganglion cells (RGCs), is regulated by cell-intrinsic determinants and cell-to-cell signaling events. The basic-helix-loop-helix (bHLH) protein Atoh7 is a key neurogenic transcription factor required for RGC development. Here, we investigate whether manipulating human ATOH7 expression among uncommitted progenitors can promote RGC fate specification and thus be used as a strategy to enhance RGC genesis. Using the chicken retina as a model, we show that cell autonomous expression of ATOH7 is sufficient to induce precocious RGC formation and expansion of the neurogenic territory. ATOH7 overexpression among neurogenic progenitors significantly enhances RGC production at the expense of reducing the progenitor pool. Furthermore, forced expression of ATOH7 leads to a minor increase of cone photoreceptors. We provide evidence that elevating ATOH7 levels accelerates cell cycle progression from S to M phase and promotes cell cycle exit. We also show that ATOH7-induced ectopic RGCs often exhibit aberrant axonal projection patterns and are correlated with increased cell death during the period of retinotectal connections. These results demonstrate the high potency of human ATOH7 in promoting early retinogenesis and specifying the RGC differentiation program, thus providing insight for manipulating RGC production from stem cell-derived retinal organoids.

Indexed as

AnimalsBasic Helix-Loop-Helix ProteinsCell Cycle CheckpointsCell DifferentiationCell ProliferationCell SurvivalChick EmbryoGenetic VectorsHumansModels, AnimalNeurogenesisPhotoreceptor Cells, VertebrateRetinal Ganglion CellsRetroviridaeStem CellsATOH7 protein, humanBasic Helix-Loop-Helix Proteins

Identifiers

PMID29717171
PMCPMC5931526
OpenAlexW2801417830

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.