ArticleJournal of ovarian research2018
Successful fertility following optimized perfusion and cryopreservation of whole ovary and allotransplantation in a premature ovarian insufficiency rat model.
Article in Journal of ovarian research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Cryopreservation of ovarian tissue - what's known so far and future perspectives.Frontiers in reproductive health · 2026Review
- Cryopreservation of Rodent Ovarian Tissues and Oocytes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- 55 MHz constant field dielectric warming of kidneys and ovaries cryopreserved by vitrification.Cryobiology · 2025Article
- Identification of genetically engineered strategies to manipulate nano-platforms presenting immunotherapeutic ligands for alleviating primary ovarian insufficiency progression.Cell communication and signaling : CCS · 2025Article
- Effect of Donor Age on Endocrine Function of and Immune Response to Ovarian Grafts.International journal of molecular sciences · 2024Article
- Accelerating cryoprotectant delivery using vacuum infiltration.Cryobiology · 2023Article
- Current Animal Model Systems for Ovarian Aging Research.Aging and disease · 2022Review
- Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model.BMC biotechnology · 2021Article
- Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans.Journal of clinical medicine · 2020Review
- Hypothermic machine perfusion after static cold storage improves ovarian function in rat ovarian tissue transplantation.Journal of assisted reproduction and genetics · 2020Article
- Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF.PloS one · 2020Article
- Current Progress in Uterus Transplantation Research in Asia.Journal of clinical medicine · 2019Review
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
backgroundFertility preservation by whole ovary cryopreservation and transplantation (WOCP&TP) with vascular anastomosis requires successful cryopreservation. In this study, we investigated the possibility of restoring ovarian function and natural fertility after WOCP&TP in a premature ovarian insufficiency (POI) rat model. The influence of cryopreservation on the offspring of rats following WOCP&TP was also explored.
methodRats aged 8-10 weeks were used as donors and recipients for allotransplantation. Fifteen rat whole ovaries were divided into three groups: the optimized group, the conventional group, and the fresh group. Different perfusion modes were used before cryopreservation and after thawing. Whole ovaries were observed by morphologic analysis, immunohistochemical staining, and transferase-mediated deoxyuridine triphosphate nick end-labeling assay. Ovarian function and fertility after WOCP&TP were then observed in 25 cyclophosphamide-induced POI rats for 8 months. Ovarian function was assessed by vaginal smears and blood hormone levels. Fertility restoration was quantified as the live birth rate after mating. The filial generation of rats was mated at 8-10 weeks of age. Offspring were observed for birth defect.
resultsHistological evaluation demonstrated intact morphology of follicles in all groups, with 77.6% of the total number of follicles identified as intact in the optimized group. The apoptotic rates of ovarian cells in the optimized group were significantly lower than that in the conventional group. Of the 20 live POI rats, 14 (70%) began to recover ovarian function after 2 weeks of transplantation, with normal hormone levels achieved 4 weeks after transplantation. Four of 14 rats were pregnant and delivered live offspring. One rat had a second pregnancy and delivered a second litter of live offspring. When the offspring matured, they were mated, and second and third generations of rats were born. All offspring had no abnormalities in appearance.
conclusionsHigh rates of restoration of ovarian function and natural fertility with multiple generations of offspring were obtained following WOCP&TP in a cyclophosphamide-induced POI rat model by utilizing optimized perfusion. Cryopreservation did not affect the viability of successive generations.
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