Evidence map›Paper›PMID 29716583›Full record

ArticleBMC veterinary research2018

Immunization with one Theileria parva strain results in similar level of CTL strain-specificity and protection compared to immunization with the three-component Muguga cocktail in MHC-matched animals.

Lucilla Steinaa, Nicholas Svitek, Elias Awino, Thomas Njoroge, Rosemary Saya, Ivan Morrison, Philip Toye

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Comparison of theParasitology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Lucilla SteinaaInternational Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya. l.steinaa@cgiar.org.ORCID http://orcid.org/0000-0003-3691-3971
Nicholas SvitekInternational Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya.
Elias AwinoInternational Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya.
Thomas NjorogeInternational Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya.
Rosemary SayaInternational Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya.
Ivan MorrisonThe Roslin Institute, The University of Edinburgh, Midlothian, EH25 9RG, UK.
Philip ToyeInternational Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya.
International Livestock Research Institute · KERoslin Institute · GB

Funding

Biotechnology and Biological Sciences Research Council BB/H009515/1
6 · The paper itself

Abstract

backgroundThe tick-borne protozoan parasite Theileria parva causes a usually fatal cattle disease known as East Coast fever in sub-Saharan Africa, with devastating consequences for poor small-holder farmers. Immunity to T. parva, believed to be mediated by a cytotoxic T lymphocyte (CTL) response, is induced following natural infection and after vaccination with a live vaccine, known as the Infection and Treatment Method (ITM). The most commonly used version of ITM is a combination of parasites derived from three isolates (Muguga, Kiambu 5 and Serengeti-transformed), known as the "Muguga cocktail". The use of a vaccine comprising several strains is believed to be required to induce a broad immune response effective against field challenge. In this study we investigated whether immunization with the Muguga cocktail induces a broader CTL response than immunization with a single strain (Muguga).

resultsFour MHC haplotype-matched pairs of cattle were immunized with either the trivalent Muguga cocktail or the single Muguga strain. CTL specificity was assessed on a panel of five different strains, and clonal responses to these strains were also assessed in one of the MHC-matched pairs. We did not find evidence for a broader CTL response in animals immunized with the Muguga cocktail compared to those immunized with the Muguga strain alone, in either the bulk or clonal CTL analyses. This was supported by an in vivo trial in which all vaccinated animals survived challenge with a lethal dose of the Muguga cocktail vaccine stabilate.

conclusionWe did not observe any substantial differences in the immunity generated from animals immunized with either Muguga alone or the Muguga cocktail in the animals tested here, corroborating earlier results showing limited antigenic diversity in the Muguga cocktail. These results may warrant further field studies using single T. parva strains as future vaccine candidates.

Indexed as

AnimalsCattleGenes, MHC Class IHaplotypesMajor Histocompatibility ComplexProtozoan VaccinesSpecies SpecificityTheileria parvaTheileriasisT-Lymphocytes, CytotoxicProtozoan VaccinesCytotoxic T cellsImmunityLive vaccineStrain specificityTheileria parva

Identifiers

PMID29716583
PMCPMC5930519
OpenAlexW2805809584

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.