Evidence map›Paper›PMID 29714690›Full record

ArticleeLife2018

Multivalency regulates activity in an intrinsically disordered transcription factor.

Sarah Clark, Janette B Myers, Ashleigh King, Radovan Fiala, Jiri Novacek, Grant Pearce, Jörg Heierhorst, Steve L Reichow, Elisar J Barbar

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.6field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 51 citations in OpenAlex.

  1. Biomolecular NMR assignments · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

Sarah ClarkDepartment of Biochemistry and Biophysics, Oregon State University, Oregon, United States.
Janette B MyersDepartment of Chemistry, Portland State University, Oregon, United States.ORCID http://orcid.org/0000-0002-7758-2649
Ashleigh KingSt. Vincent's Institute of Medical Research, The University of Melbourne, Victoria, Australia.
Radovan FialaCentral European Institute of Technology, Masaryk University, Brno, Czech Republic.
Jiri NovacekCentral European Institute of Technology, Masaryk University, Brno, Czech Republic.
Grant PearceSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.ORCID http://orcid.org/0000-0002-2683-0331
Jörg HeierhorstSt. Vincent's Institute of Medical Research, The University of Melbourne, Victoria, Australia.ORCID http://orcid.org/0000-0003-2789-9514
Steve L ReichowDepartment of Chemistry, Portland State University, Oregon, United States.
Elisar J BarbarDepartment of Biochemistry and Biophysics, Oregon State University, Oregon, United States.ORCID http://orcid.org/0000-0003-4892-5259
Central European Institute of Technology · CZOregon State University · USPortland State University · USThe University of Melbourne · AUUniversity of Canterbury · NZ

Funding

Dynamic Mechanisms of Membrane Channel Gating by CryoEMR35GM124779 · NIGMS · PORTLAND STATE UNIVERSITY · PI Stephen Loen Reichow · 2017 to 2026
$4.2M
Dynein light chain as a dimerization hub for natively disordered proteinsR01GM084276 · NIGMS · OREGON STATE UNIVERSITY · PI BARBAR, ELISAR J · 2009 to 2013
$1.5M
Acquisition of a 700 MHz NMR SpectrometerS10OD018518 · OD · OREGON STATE UNIVERSITY · PI BARBAR, ELISAR J · 2015 to 2015
$1.3M
NIGMS NIH HHS R01-084276NIGMS NIH HHS R01 GM084276NIGMS NIH HHS R35 GM124779NIGMS NIH HHS R35GM124779NIH HHS 1S10OD018518NIH HHS S10 OD018518
6 · The paper itself

Abstract

The transcription factor ASCIZ (ATMIN, ZNF822) has an unusually high number of recognition motifs for the product of its main target gene, the hub protein LC8 (DYNLL1). Using a combination of biophysical methods, structural analysis by NMR and electron microscopy, and cellular transcription assays, we developed a model that proposes a concerted role of intrinsic disorder and multiple LC8 binding events in regulating LC8 transcription. We demonstrate that the long intrinsically disordered C-terminal domain of ASCIZ binds LC8 to form a dynamic ensemble of complexes with a gradient of transcriptional activity that is inversely proportional to LC8 occupancy. The preference for low occupancy complexes at saturating LC8 concentrations with both human and

Indexed as

Gene Expression RegulationAnimalsCytoplasmic DyneinsDrosophila melanogasterDrosophila ProteinsDyneinsHumansIntrinsically Disordered ProteinsTranscription FactorsATMIN protein, humanctp protein, DrosophilaCytoplasmic DyneinsDrosophila ProteinsDyneinsDYNLL1 protein, humanIntrinsically Disordered ProteinsTranscription Factorsdynamic complexeselectron microscopyhumanIntrinsically disordered proteinsmolecular biophysicsmultivalencyNMRstructural biologytranscription factors

Identifiers

PMID29714690
PMCPMC5963919
OpenAlexW2799698455

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.