ArticleNature methods2018
Self-interference 3D super-resolution microscopy for deep tissue investigations.
Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 113 citations in OpenAlex.
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- X-ray multiscale 3D neuroimaging to quantify cellular aging and neurodegeneration postmortem in a model of Alzheimer's disease.European journal of nuclear medicine and molecular imaging · 2022Article
- Rapid 3D-STORM imaging of diverse molecular targets in tissue.Cell reports methods · 2022Article
- Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer.Science advances · 2022Article
- Transcription factor dynamics in plants: Insights and technologies for in vivo imaging.Plant physiology · 2022Review
- When Super-Resolution Localization Microscopy Meets Carbon Nanotubes.Nanomaterials (Basel, Switzerland) · 2022Review
- Present Application and Perspectives of Organoid Imaging Technology.Bioengineering (Basel, Switzerland) · 2022Review
- Super-resolution imaging: when biophysics meets nanophotonics.Nanophotonics (Berlin, Germany) · 2022Review
- PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells.Nature communications · 2021Article
- Roadmap on Recent Progress in FINCH Technology.Journal of imaging · 2021Review
- Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques.Science China. Life sciences · 2021Review
- Robust adaptive optics for localization microscopy deep in complex tissue.Nature communications · 2021Article
- Super-resolution imaging of flat-mounted whole mouse cornea.Experimental eye research · 2021Article
- Axial localization and tracking of self-interference nanoparticles by lateral point spread functions.Nature communications · 2021Article
Corrections and comments
- Commented on byLaurent Cognet.2018
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence localization microscopy has achieved near-molecular resolution capable of revealing ultra-structures, with a broad range of applications, especially in cellular biology. However, it remains challenging to attain such resolution in three dimensions and inside biological tissues beyond the first cell layer. Here we introduce SELFI, a framework for 3D single-molecule localization within multicellular specimens and tissues. The approach relies on self-interference generated within the microscope's point spread function (PSF) to simultaneously encode equiphase and intensity fluorescence signals, which together provide the 3D position of an emitter. We combined SELFI with conventional localization microscopy to visualize F-actin 3D filament networks and reveal the spatial distribution of the transcription factor OCT4 in human induced pluripotent stem cells at depths up to 50 µm inside uncleared tissue spheroids. SELFI paves the way to nanoscale investigations of native cellular processes in intact tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.