ArticleNature cell biology2018
A PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cells.
Article in Nature cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Whole-Genome Sequencing Reveals the Role of Cis-Regulatory Elements and eQTL/sQTL in the Adaptive Selection of Hubei Indigenous Cattle.Animals : an open access journal from MDPI · 2025Article
- The establishment and regulation of human germ cell lineage.Stem cell research & therapy · 2025Review
- The role of KLF4 in human primordial germ cell development.Open biology · 2025Article
- DDX5 Can Act as a Transcription Factor Participating in the Formation of Chicken PGCs by TargetingGenes · 2024Article
- From in vivo to in vitro: exploring the key molecular and cellular aspects of human female gametogenesis.Human cell · 2023Review
- Immune and spermatogenesis-related loci are involved in the development of extreme patterns of male infertility.Communications biology · 2022Article
- Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation.Protein & cell · 2022Article
- Review
- Early Gonadal Development and Sex Determination in Mammal.International journal of molecular sciences · 2022Review
- Transcriptional control of human gametogenesis.Human reproduction update · 2022Article
- The Blimp-1 transcription factor acts in non-neuronal cells to regulate terminal differentiation of the Drosophila eye.Development (Cambridge, England) · 2022Article
- NBMA Promotes Spermatogenesis by Mediating Oct4 Pathway.ChemistryOpen · 2022Article
- Germline specification from pluripotent stem cells.Stem cell research & therapy · 2022Review
- Direct reprogramming of human Sertoli cells into male germline stem cells with the self-renewal and differentiation potentials via overexpressing DAZL/DAZ2/BOULE genes.Stem cell reports · 2021Article
- Characterizing the Low-Dose Effects of Methylmercury on the Early Stages of Embryo Development Using Cultured Human Embryonic Stem Cells.Environmental health perspectives · 2021Article
- Recent advances: fertility preservation and fertility restoration options for males and females.Faculty reviews · 2021Review
- Chemical Genetics: Manipulating the Germline with Small Molecules.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Evaluation of Male Fertility-Associated Loci in a European Population of Patients with Severe Spermatogenic Impairment.Journal of personalized medicine · 2020Article
- Transcription factor AP2 controls cnidarian germ cell induction.Science (New York, N.Y.) · 2020Article
- Characterization and Isolation of Very Small Embryonic-like (VSEL) Stem Cells Obtained from Various Human Hematopoietic Cell Sources.Stem cell reviews and reports · 2019Article
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
Dysregulation of genetic pathways during human germ cell development leads to infertility. Here, we analysed bona fide human primordial germ cells (hPGCs) to probe the developmental genetics of human germ cell specification and differentiation. We examined the distribution of OCT4 occupancy in hPGCs relative to human embryonic stem cells (hESCs). We demonstrated that development, from pluripotent stem cells to germ cells, is driven by switching partners with OCT4 from SOX2 to PAX5 and PRDM1. Gain- and loss-of-function studies revealed that PAX5 encodes a critical regulator of hPGC development. Moreover, an epistasis analysis indicated that PAX5 acts upstream of OCT4 and PRDM1. The PAX5-OCT4-PRDM1 proteins form a core transcriptional network that activates germline and represses somatic programmes during human germ cell differentiation. These findings illustrate the power of combined genome editing, cell differentiation and engraftment for probing human developmental genetics that have historically been difficult to study.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.