ArticleGenome research2018
Arrayed CRISPR screen with image-based assay reliably uncovers host genes required for coxsackievirus infection.
Article in Genome research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 59 citations in OpenAlex.
- CRISPR/Cas9 loss-of-function screen in a neuronal model of AP-4 deficiency identifies ATG9A trafficking modulators.JCI insight · 2026Article
- A Comprehensive Review on CRISPR-Based Screening and Its Applications.Molecular biotechnology · 2026Review
- CSDE1 promotes viral immune evasion through RNA-dependent and phosphorylation-modulated liquid-liquid phase separation.Nature communications · 2026Article
- Insights from pooled CRISPRi single-cell screens in K562 cells reveal gene functions, regulatory networks, and highlight opportunities and limitations.BMC genomics · 2026Article
- Functional Genomics: Principles and Methods.Advances in experimental medicine and biology · 2026Review
- Integrating AUROC and SSMD for quality control in high-throughput screening assays.SLAS discovery : advancing life sciences R & D · 2025Article
- Review
- A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist.PLoS biology · 2024Article
- Identification of the proteolytic signature in CVB3-infected cells.Journal of virology · 2024Article
- Genome-scale requirements for dynein-based transport revealed by a high-content arrayed CRISPR screen.The Journal of cell biology · 2024Article
- Recent advances in CRISPR-based functional genomics for the study of disease-associated genetic variants.Experimental & molecular medicine · 2024Review
- Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection.Nature communications · 2024Article
- A statistical simulation model to guide the choices of analytical methods in arrayed CRISPR screen experiments.PloS one · 2024Article
- Applying CRISPR-Cas9 screens to dissect hematological malignancies.Blood advances · 2023Review
- Genome-scale requirements for dynein-based trafficking revealed by a high-content arrayed CRISPR screen.bioRxiv : the preprint server for biology · 2023Article
- Role of the receptor for activated C kinase 1 during viral infection.Archives of virology · 2022Review
- Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection.Research square · 2022Article
- Pooled genetic perturbation screens with image-based phenotypes.Nature protocols · 2022Article
- Genome-Wide Mutant Screening in Yeast Reveals that the Cell Wall is a First Shield to Discriminate Light From Heavy Lanthanides.Frontiers in microbiology · 2022Article
- Cellular host factors for SARS-CoV-2 infection.Nature microbiology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pooled CRISPR screens based on lentiviral systems have been widely applied to identify the effect of gene knockout on cellular phenotype. Although many screens were successful, they also have the limitation that genes conferring mild phenotypes or those essential for growth can be overlooked, as every genetic perturbation is incorporated in the same population. Arrayed screens, on the other hand, incorporate a single genetic perturbation in each well and could overcome these limitations. However, arrayed screens based on siRNA-mediated knockdown were recently criticized for low reproducibility caused by incomplete inhibition of gene expression. To overcome these limitations, we developed a novel arrayed CRISPR screen based on a plasmid library expressing a single guide RNA (sgRNA) and disrupted 1514 genes, encoding kinases, proteins related to endocytosis, and Golgi-localized proteins, individually using 4542 sgRNAs (three sgRNAs per gene). This screen revealed host factors required for infection by coxsackievirus B3 (CVB3) from
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