ArticleBMC biology2018
Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation.
Article in BMC biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
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- Optical inactivation of intracellular molecules by fast-maturating photosensitizing fluorescence protein, HyperNova.Communications biology · 2024Article
- Chromophore-Assisted Light Inactivation for Protein Degradation and Its Application in Biomedicine.Bioengineering (Basel, Switzerland) · 2024Review
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Chromophore-assisted light inactivation of target proteins for singularity biology.Biophysics and physicobiology · 2024Review
- Chemical biology approaches to uncovering nuclear ROS control.Current opinion in chemical biology · 2023Review
- Optogenetic polymerization and assembly of electrically functional polymers for modulation of single-neuron excitability.Science advances · 2022Article
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
- Optogenetic activators of apoptosis, necroptosis, and pyroptosis.The Journal of cell biology · 2022Article
- Optogenetic Control of Non-Apoptotic Cell Death.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Tailoring photosensitive ROS for advanced photodynamic therapy.Experimental & molecular medicine · 2021Review
- Optical manipulation of molecular function by chromophore-assisted light inactivation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2021Review
- Genetically Encoded Photosensitizer for Destruction of Protein or Cell Function.Advances in experimental medicine and biology · 2021Review
- Photodynamic Activation of Cholecystokinin 1 Receptor with Different Genetically Encoded Protein Photosensitizers and from Varied Subcellular Sites.Biomolecules · 2020Article
- Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems.Cell chemical biology · 2020Article
- Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation.Scientific reports · 2020Article
- Molecular Tools to Generate Reactive Oxygen Species in Biological Systems.Bioconjugate chemistry · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhotosensitizing fluorescent proteins, which generate reactive oxygen species (ROS) upon light irradiation, are useful for spatiotemporal protein inactivation and cell ablation. They give us clues about protein function, intracellular signaling pathways and intercellular interactions. Since ROS generation of a photosensitizer is specifically controlled by certain excitation wavelengths, utilizing colour variants of photosensitizing protein would allow multi-spatiotemporal control of inactivation. To expand the colour palette of photosensitizing protein, here we developed SuperNova Green from its red predecessor, SuperNova.
resultsSuperNova Green is able to produce ROS spatiotemporally upon blue light irradiation. Based on protein characterization, SuperNova Green produces insignificant amounts of singlet oxygen and predominantly produces superoxide and its derivatives. We utilized SuperNova Green to specifically inactivate the pleckstrin homology domain of phospholipase C-δ1 and to ablate cancer cells in vitro. As a proof of concept for multi-spatiotemporal control of inactivation, we demonstrate that SuperNova Green can be used with its red variant, SuperNova, to perform independent protein inactivation or cell ablation studies in a spatiotemporal manner by selective light irradiation.
conclusionDevelopment of SuperNova Green has expanded the photosensitizing protein toolbox to optogenetically control protein inactivation and cell ablation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.