Evidence map›Paper›PMID 29709902›Full record

ArticleThe Journal of veterinary medical science2018

Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice.

Ai-Ping Hsu, Chun-Hsien Tseng, Yi-Ta Lu, Yu-Hua Shih, Chung-Hsi Chou, Re-Shang Chen, Kuo-Jung Tsai, Wen-Jane Tu, Florence Cliquet, Hsiang-Jung Tsai

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Ai-Ping HsuAnimal Health Research Institute, Council of Agriculture, No.376, Zhong zheng Rd., Tamsui Dist., New Taipei City 251, Taiwan (R.O.C.).
Chun-Hsien TsengAnimal Health Research Institute, Council of Agriculture, No.376, Zhong zheng Rd., Tamsui Dist., New Taipei City 251, Taiwan (R.O.C.).
Yi-Ta LuAnimal Health Research Institute, Council of Agriculture, No.376, Zhong zheng Rd., Tamsui Dist., New Taipei City 251, Taiwan (R.O.C.).
Yu-Hua ShihAnimal Health Research Institute, Council of Agriculture, No.376, Zhong zheng Rd., Tamsui Dist., New Taipei City 251, Taiwan (R.O.C.).
Chung-Hsi ChouGraduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan (R.O.C.).
Re-Shang ChenAnimal Health Research Institute, Council of Agriculture, No.376, Zhong zheng Rd., Tamsui Dist., New Taipei City 251, Taiwan (R.O.C.).
Kuo-Jung TsaiAnimal Health Research Institute, Council of Agriculture, No.376, Zhong zheng Rd., Tamsui Dist., New Taipei City 251, Taiwan (R.O.C.).
Wen-Jane TuAnimal Health Research Institute, Council of Agriculture, No.376, Zhong zheng Rd., Tamsui Dist., New Taipei City 251, Taiwan (R.O.C.).
Florence CliquetNancy OIE/WHO/EU Laboratory for Rabies and Wildlife, French Agency for Food, Environmental and Occupational Health &Safety, Technopole Agricole et Vétérinaire de Pixérécourt, Bâtiment H, CS 40009, 54220 MALZEVILLE, France.
Hsiang-Jung TsaiGraduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan (R.O.C.).
Animal Health Research Institute · TWCouncil of Agriculture · TWNational Taiwan University · TWAgence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In 2013, the first case of Taiwan ferret badger rabies virus (RABV-TWFB) infection was reported in Formosan ferret badgers, and two genetic groups of the virus were distinguished through phylogenetic analysis. To detect RABV-TWFB using a sensitive nucleic acid-based method, a quantitative real-time reverse transcription polymerase chain reaction targeting the conserved region of both genetic groups of RABV-TWFB was developed. This method had a limit of detection (LOD) of 40 RNA copies/reaction and detected viral RNA in brain and ear tissue specimens of infected and dead Formosan ferret badgers and mice with 100% sensitivity and specificity. The mean viral RNA load detected in the ear tissue specimens of ferret badgers ranged from 3.89 × 10

Indexed as

AnimalsFerretsMiceMice, Inbred BALB CMustelidaePhylogenyRabiesRabies virusReverse Transcriptase Polymerase Chain ReactionRNA, ViralTaiwanRNA, Viralrabies diagnosisreal-time RT-PCRTaiwan ferret badger rabies

Identifiers

PMID29709902
PMCPMC6021896
OpenAlexW2802877837

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.