ArticlePeerJ2018
A robust method for RNA extraction and purification from a single adult mouse tendon.
Article in PeerJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Regulators of ECM structure enable functional adaptation to tensile loading in tendon explants.Matrix biology : journal of the International Society for Matrix Biology · 2026Article
- Prolyl-3-hydroxylase 1 is a central regulator of collagen post-translational modifications and the collagen biosynthetic network.The Journal of biological chemistry · 2026Article
- Regulators of ECM Structure Enable Functional Adaptation to Tensile Loading in Tendon Explants.bioRxiv : the preprint server for biology · 2026Article
- Comparative Analysis of Tendon Healing in a Clinically Relevant Mouse Model of Achilles Tendon Repair.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Transcriptional Adaptations to Muscle Loading in a Murine Model of Achilles Tendinopathy.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Dynamic transcriptional and epigenetic changes define postnatal tendon growth.PLoS genetics · 2025Article
- Fascicular Elastin Impacts Tendon-Specific Ramp-to-Failure and Fatigue Mechanics.Journal of biomechanical engineering · 2025Article
- Urinary sodium wasting and disrupted collecting duct function in mice with distal renal tubular acidosis mutations.Disease models & mechanisms · 2025Article
- Cellular senescence impairs tendon extracellular matrix remodeling in response to mechanical unloading.Aging cell · 2024Article
- A latent Axin2NPJ Regenerative medicine · 2024Article
- Aged Tendons Exhibit Altered Mechanisms of Strain-Dependent Extracellular Matrix Remodeling.Journal of biomechanical engineering · 2024Article
- Preclinical tendon and ligament models: Beyond the 3Rs (replacement, reduction, and refinement) to 5W1H (why, who, what, where, when, how).Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Review
- Viable tendon neotissue from adult adipose-derived multipotent stromal cells.Frontiers in bioengineering and biotechnology · 2023Article
- Research perspectives-Pipelines to human tendon transcriptomics.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Review
- Advances in single-cell sequencing and its application to musculoskeletal system research.Cell proliferation · 2022Review
- Bringing tendon biology to heel: Leveraging mechanisms of tendon development, healing, and regeneration to advance therapeutic strategies.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
- Comparison of Snail1, ZEB1, E-Cadherin Expression Levels in HPV-Induced Cervical Cancer.Iranian journal of public health · 2020Article
- Widespread diversity in the transcriptomes of functionally divergent limb tendons.The Journal of physiology · 2020Article
- Adaptive and innate immune cell responses in tendons and lymph nodes after tendon injury and repair.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- RNA-seq in Skeletal Biology.Current osteoporosis reports · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMechanistic understanding of tendon molecular and cellular biology is crucial toward furthering our abilities to design new therapies for tendon and ligament injuries and disease. Recent transcriptomic and epigenomic studies in the field have harnessed the power of mouse genetics to reveal new insights into tendon biology. However, many mouse studies pool tendon tissues or use amplification methods to perform RNA analysis, which can significantly increase the experimental costs and limit the ability to detect changes in expression of low copy transcripts.
methodsSingle Achilles tendons were harvested from uninjured, contralateral injured, and wild type mice between three and five months of age, and RNA was extracted. RNA Integrity Number (RIN) and concentration were determined, and RT-qPCR gene expression analysis was performed.
resultsAfter testing several RNA extraction approaches on single adult mouse Achilles tendons, we developed a protocol that was successful at obtaining high RIN and sufficient concentrations suitable for RNA analysis. We found that the RNA quality was sensitive to the time between tendon harvest and homogenization, and the RNA quality and concentration was dependent on the duration of homogenization. Using this method, we demonstrate that analysis of DISCUSSION: Our work presents a robust, cost-effective, and straightforward method to extract high quality RNA from a single adult mouse Achilles tendon at sufficient amounts for RT-qPCR as well as RNA-seq. We show this can reduce variation and decrease the overall costs associated with experiments. This approach can also be applied to other skeletal tissues, as well as precious human samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.