ArticleStructure (London, England : 1993)2018
Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.
Article in Structure (London, England : 1993), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
116 citing papers in PubMed.
- Characterization of energy filtering slit widths for MicroED data collection.Journal of structural biology: X · 2026Article
- Atomic resolution structure of human papillomavirus bound to heparin.Nature communications · 2026Article
- Atomic resolution cryo-EM at 200 keV.IUCrJ · 2026Article
- Anticodon loop remodeling and D-stem shape drive the specific recognition of ANN-decoding tRNAs for t6A modification.Nucleic acids research · 2026Article
- Tracking the redox reaction of the iron enzyme ribonucleotide reductase using continuous SerialED and SFX.Structure (London, England : 1993) · 2026Article
- Membrane protein structure and dynamics probed by MicroED.Biochemical Society transactions · 2026Review
- Structure and mechanism of the heterotetrameric ADP-glucose pyrophosphorylase essential for starch synthesis in plants.Science advances · 2026Article
- Quantum refinement with electron diffraction and X-ray free-electron laser data: comparative study of ribonucleotide reductase dimetal site.Journal of applied crystallography · 2026Article
- Direct evidence of acid-driven protein desolvation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Direct from the seed: an atomic resolution protein structure by ab initio MicroED.Nature communications · 2026Article
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Limiting the effects of radiation damage in MicroED through dose selection during data processing.Acta crystallographica. Section D, Structural biology · 2025Article
- High-Throughput MicroED for Probing Ion Channel Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- Recovering high-resolution information using energy filtering in MicroED.Structural dynamics (Melville, N.Y.) · 2025Article
- Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structure of the lens MP20 mediated adhesive junction.Nature communications · 2025Article
- Energy filtering enables macromolecular MicroED data at sub-atomic resolution.Nature communications · 2025Article
- Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination.IUCrJ · 2025Article
56 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data.
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Registered trials
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