ArticleTranslational oncology2018
Method Optimization for Extracting High-Quality RNA From the Human Pancreas Tissue.
Article in Translational oncology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Improved stabilisation of human pancreas biopsies: Comparative evaluation of RNA preservation methods.World journal of biological chemistry · 2026Article
- Pancreatic injury induces β-cell regeneration in axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Use of organ transplant solution to preserve skeletal muscle for cellular and spatial transcriptomic analyses.Scientific reports · 2026Article
- Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes.BMC biotechnology · 2025Article
- A protocol for microRNA extraction from gastrointestinal digesta.Food chemistry. Molecular sciences · 2025Article
- Transcriptome-wide m6A landscape across diverse porcine tissues revealed by nanopore direct RNA sequencing.Frontiers in genetics · 2025Article
- Melatonin Receptors and Serotonin: Age-Related Changes in the Ovaries.Journal of personalized medicine · 2024Article
- Inflammaging: Expansion of Molecular Phenotype and Role in Age-Associated Female Infertility.Biomedicines · 2024Article
- The Expression of Kisspeptins and Matrix Metalloproteinases in Extragenital Endometriosis.Biomedicines · 2024Article
- Article
- Integrated analysis revealed hypoxia signatures and LDHA related to tumor cell dedifferentiation and unfavorable prognosis in pancreatic adenocarcinoma: Hypoxia in PDAC.Translational oncology · 2023Article
- Highly efficient cell-microbead encapsulation using dielectrophoresis-assisted dual-nanowell array.PNAS nexus · 2023Article
- An optimized method for high-quality RNA extraction from distinctive intrinsic laryngeal muscles in the rat model.Scientific reports · 2022Article
- Refining the Molecular Framework for Pancreatic Cancer with Single-cell and Spatial Technologies.Clinical cancer research : an official journal of the American Association for Cancer Research · 2021Review
- The guanidine thiocyanate-high EDTA method for total microbial RNA extraction from severely heavy metal-contaminated soils.Microbial biotechnology · 2021Article
- Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing.BMC genomics · 2021Article
- Gene Expression Analysis of the Pre-Diabetic Pancreas to Identify Pathogenic Mechanisms and Biomarkers of Type 1 Diabetes.Frontiers in endocrinology · 2020Article
- Extraction of high-quality RNA from mouse pancreatic tumors.MethodsX · 2020Article
- In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network.Journal of translational medicine · 2019Review
- Comprehensive proteome and phosphoproteome profiling shows negligible influence of RNAlater on protein abundance and phosphorylation.Clinical proteomics · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid sequencing is frequently used to determine the molecular basis of diseases. Therefore, proper storage of biological specimens is essential to inhibit nucleic acid degradation. RNA isolated from the human pancreas is generally of poor quality because of its high concentration of endogenous RNase. In this study, we optimized the method for extracting high quality RNA from paired tumor and normal pancreatic tissues obtained from eight pancreatic cancer patients post-surgery. RNA integrity number (RIN) was checked to evaluate the integrity of RNA, we tried to extract the RNA with an RIN value of 8 or higher that allows for the latest genetic analysis. The effect of several parameters, including the method used for tissue lysis, RNAlater treatment, tissue weight at storage, and the time to storage after surgical resection, on the quantity and quality of RNA extracted was examined. Data showed that the highest quantity of RNA was isolated using a combination of manual and mechanical methods of tissue lysis. Additionally, sectioning the tissues into small pieces (<100 mg) and treating them with RNAlater solution prior to storage increased RNA stability. Following these guidelines, high quality RNA was obtained from 100% (8/8) of tumor tissues and 75% (6/8) of normal tissues. High-quality RNA was still stable under repeated freezing and thawing. The application of these results during sample handling and storage in clinical settings will facilitate the genetic diagnosis of diseases and their subsequent treatment.
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Registered trials
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