Evidence map›Paper›PMID 29705183›Full record

ArticleMolecular and cellular probes2018

Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2.

Libing Liu, Jianchang Wang, Yunyun Geng, Jinfeng Wang, Ruiwen Li, Ruihan Shi, Wanzhe Yuan

Open access · greenAbstract read
In one paragraph

Article in Molecular and cellular probes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 20 citations in OpenAlex.

  1. Molecular Detection of Feline HemotropicVeterinary sciences · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Rapid Detection ofFrontiers in cellular and infection microbiology · 2022
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2.Chinese chemical letters = Zhongguo hua xue kuai bao · 2019
    Article
  11. Article
  12. Article
  13. Review
  14. Rapid and Specific Detection ofFrontiers in microbiology · 2019
    Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Libing LiuCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Jianchang WangCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Yunyun GengCollege of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
Jinfeng WangCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Ruiwen LiCollege of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.
Ruihan ShiCenter of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China.
Wanzhe YuanCollege of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China. Electronic address: yuanwanzhe2015@126.com.
Entry Exit Inspection and Quarantine Bureau · CNHebei Agricultural University · CNHebei Food Inspection and Research Institute · CNHebei Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A visible and equipment-free recombinase polymerase amplification assay combined with a lateral flow strip (LFS RPA) was developed to detect canine parvovirus type 2 (CPV-2), which is the etiological agent of canine parvovirus disease. The CPV-2 LFS RPA assay was developed based on the VP2 gene and is performed in a closed fist using body heat for 15 min; the products are visible to the naked eye on the LFS within 5 min. The assay could detect CPV-2a, CPV-2b and CPV-2c, and there was no cross-reaction with the other viruses tested. Using the standard CPV-2 DNA as a template, the analytical sensitivity was 1.0 × 10

Indexed as

Hot TemperatureAnimalsDNA, ViralDog DiseasesDogsParvovirus, CaninePolymerase Chain ReactionRecombinasesRheologySensitivity and SpecificityTime FactorsDNA, ViralRecombinasesCPV-2Lateral flow stripnfo probeRPAVP2 gene

Identifiers

PMID29705183
PMCPMC7127419
OpenAlexW2799440413

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.