Evidence map›Paper›PMID 29705001›Full record

ArticleBiomaterials2018

Dendritic peptide bolaamphiphiles for siRNA delivery to primary adipocytes.

Alexander C Eldredge, Mark E Johnson, Yang Cao, Lin Zhang, Can Zhao, Zhengxia Liu, Qin Yang, Zhibin Guan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Alexander C EldredgeDepartment of Chemistry, University of California, Irvine, CA 92697, United States.
Mark E JohnsonDepartment of Chemistry, University of California, Irvine, CA 92697, United States.
Yang CaoDepartment of Medicine, Physiology & Biophysics, University of California, Irvine, CA 92697, United States.
Lin ZhangDepartment of Medicine, Physiology & Biophysics, University of California, Irvine, CA 92697, United States.
Can ZhaoDepartment of Medicine, Physiology & Biophysics, University of California, Irvine, CA 92697, United States; Department of Geriatrics, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 211166, China.
Zhengxia LiuDepartment of Medicine, Physiology & Biophysics, University of California, Irvine, CA 92697, United States; Department of Geriatrics, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 211166, China.
Qin YangDepartment of Medicine, Physiology & Biophysics, University of California, Irvine, CA 92697, United States. Electronic address: qin.yang@uci.edu.
Zhibin GuanDepartment of Chemistry, University of California, Irvine, CA 92697, United States; Department of Biomedical Engineering, University of California, Irvine, CA 92697, United States. Electronic address: zguan@uci.edu.
University of California, Irvine · USSecond Affiliated Hospital of Nanjing Medical University · CN

Funding

Nicotinamide N-methyltransferase is a novel regulator of energy expenditureR01DK100385 · NIDDK · UNIVERSITY OF CALIFORNIA-IRVINE · PI YANG, QIN · 2014 to 2018
$1.7M
Dynamic Dendronized Polymers for Effective siRNA Delivery to Pancreatic IsletsR01DK098446 · NIDDK · UNIVERSITY OF CALIFORNIA-IRVINE · PI GUAN, ZHIBIN · 2013 to 2015
$1.2M
NIDDK NIH HHS R01 DK098446NIDDK NIH HHS R01 DK100385
6 · The paper itself

Abstract

Obesity is a major risk factor for diabetes, heart disease and other health problems. Adipose tissue plays a central role in the development of obesity and obesity-associated diseases. Gene therapy targeting adipose tissue may provide a promising strategy for obesity treatment. However, nucleic acid delivery to adipose tissue or even cultured adipocytes is challenging due to low delivery efficacy and high toxicity of the current cationic lipid based delivery systems, or monoamphiphiles. Herein, we report using dendritic peptide bolaamphiphiles (bolas) to deliver siRNA to primary adipocytes and hepatocytes. The bola consists of two l-Lysine dendrons connected to a fluorocarbon core through disulfide linkages. The Lysine dendrons are functionalized with l-histidine and l-tryptophan to promote endosomal escape and cellular uptake. The bola exhibited over 70% knockdown of GAPDH gene in both primary adipocytes and hepatocytes. Importantly, different from Lipofectamine that significantly reduced genes involved in lipolysis, lipogenesis, fatty acid oxidation and ketogenesis, the bolas had little to no effect on these genes. These results demonstrate the bola as a promising new vector for clinical and experimental applications for delivery of siRNA to metabolic organs.

Indexed as

Gene Transfer Techniques3T3-L1 CellsAdipocytesAnimalsCell DifferentiationCells, CulturedColloidsFuransHepatocytesHumansKidneyLipid MetabolismLiverMaleMiceMice, Inbred C57BLbolaamphiphileColloidsFuransPeptidesPyridonesRNA, Small InterferingAdipocyteBolaamphiphilePrimary cellssiRNA delivery

Identifiers

PMID29705001
PMCPMC6054800
OpenAlexW2800248585

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.