Evidence map›Paper›PMID 29704030›Full record

ArticleAnalytical and bioanalytical chemistry2018

Nanospray desorption electrospray ionization mass spectrometry of untreated and treated probiotic Lactobacillus reuteri cells.

Agbo-Oma Uwakweh, Joseph N Mwangi, Daniel Todd, Zhenquan Jia, Norman H L Chiu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Agbo-Oma UwakwehDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Joseph N MwangiDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Daniel ToddDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Zhenquan JiaDepartment of Biology, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.
Norman H L ChiuDepartment of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA. prof.chiu@gmail.com.
University of North Carolina at Greensboro · US

Funding

Novel Carbon Nanodots against Vascular InflammationR15HL129212 · NHLBI · UNIVERSITY OF NORTH CAROLINA GREENSBORO · PI JIA, ZHENQUAN · 2016 to 2016
$455k
NHLBI NIH HHS R15 HL129212
6 · The paper itself

Abstract

Mass spectrometry has proven to be a useful technique for rapid identification of bacterial cells. Among various ionization techniques in mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) has been commonly used for the identification of bacterial cells. Recently, MALDI mass spectrometry has also been utilized to distinguish cellular responses. Ambient ionization techniques do support whole bacterial cell analysis, which include desorption electrospray ionization (DESI). Nanospray DESI (nDESI) is a new variant of DESI, and its application to whole-cell mass spectrometry is limited. In this project, the use of nDESI mass spectrometry to measure probiotic Lactobacillus reuteri (LR) cells is explored. A unique and reproducible mass spectral pattern of untreated LR cells was obtained by using 50% methanol/water as nDESI solvent. The use of nDESI mass spectrometry is further extended to distinguish untreated LR cells from treated LR cells that have been exposed to low pH. These findings demonstrate the feasibility of using nDESI in whole-cell mass spectrometry. Graphical abstract ᅟ.

Indexed as

Hydrogen-Ion ConcentrationLimosilactobacillus reuteriNanotechnologyProbioticsReproducibility of ResultsSolventsSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSolventsBacterial cellsNanospray DESIWhole-cell mass spectrometry

Identifiers

PMID29704030
PMCPMC6023734
OpenAlexW2802109624

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.