ArticleScientific reports2018
The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Transcriptomics informed discovery of developmentally essential transcription factors.Biology open · 2025Article
- Simulated Heat Waves Affect Cell Fate and Fitness in the Social Amoeba Dictyostelium discoideum.Microbial ecology · 2025Article
- Evolution of a novel cell type in Dictyostelia required gene duplication of a cudA-like transcription factor.Current biology : CB · 2022Article
- Autophagy of the somatic stalk cells likely nurses the propagating spores of Dictyostelid social amoebas.Open research Europe · 2022Article
- The proppin Bcas3 and its interactor KinkyA localize to the early phagophore and regulate autophagy.Autophagy · 2021Article
- Loss of PIKfyve Causes Transdifferentiation ofFrontiers in cell and developmental biology · 2021Article
- Phylogeny-wide conservation and change in developmental expression, cell-type specificity and functional domains of the transcriptional regulators of social amoebas.BMC genomics · 2019Article
- Cyclic AMP induction of Dictyostelium prespore gene expression requires autophagy.Developmental biology · 2019Article
- Cell-type specific RNA-Seq reveals novel roles and regulatory programs for terminally differentiated Dictyostelium cells.BMC genomics · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Sporulation in Dictyostelium fruiting bodies evolved from amoebozoan encystation with both being induced by cAMP acting on PKA, but with downstream components still being unknown. Using tagged mutagenesis to find missing pathway components, we identified a sporeless mutant defective in a nuclear protein, SpaA. Expression of prespore genes was strongly reduced in spaA- cells, while expression of many spore stage genes was absent. Chromatin immunoprecipitation (ChIP) of a SpaA-YFP gene fusion showed that (pre)spore gene promoters bind directly to SpaA, identifying SpaA as a transcriptional regulator. SpaA dependent spore gene expression required PKA in vivo and was stimulated in vitro by the membrane-permeant PKA agonist 8Br-cAMP. The PKA agonist also promoted SpaA binding to (pre)spore promoters, placing SpaA downstream of PKA. Sequencing of SpaA-YFP ChIPed DNA fragments revealed that SpaA binds at least 117 (pre)spore promoters, including those of other transcription factors that activate some spore genes. These factors are not in turn required for spaA expression, identifying SpaA as the major trancriptional inducer of sporulation.
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Registered trials
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