Evidence map›Paper›PMID 29703866›Full record

ArticlePlant physiology2018

Structure-Function Analysis of Chloroplast Proteins via Random Mutagenesis Using Error-Prone PCR.

Louis Dumas, Francesca Zito, Pascaline Auroy, Xenie Johnson, Gilles Peltier, Jean Alric

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. The ChloroplastCells · 2021
    Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Louis DumasLaboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Aix-Marseille Université, Unité Mixte de Recherche 7265, BIAM, Commissariat à l'Energie Atomique Cadarache, 13115 Saint-Paul-lez-Durance, France.ORCID 0000-0002-8044-7683
Francesca ZitoLaboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7099, University Paris Diderot, Sorbonne Paris Cité, PSL Research University, F-75005 Paris, France.
Pascaline AuroyLaboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Aix-Marseille Université, Unité Mixte de Recherche 7265, BIAM, Commissariat à l'Energie Atomique Cadarache, 13115 Saint-Paul-lez-Durance, France.
Xenie JohnsonLaboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Aix-Marseille Université, Unité Mixte de Recherche 7265, BIAM, Commissariat à l'Energie Atomique Cadarache, 13115 Saint-Paul-lez-Durance, France.
Gilles PeltierLaboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Aix-Marseille Université, Unité Mixte de Recherche 7265, BIAM, Commissariat à l'Energie Atomique Cadarache, 13115 Saint-Paul-lez-Durance, France.ORCID 0000-0002-2226-3931
Jean AlricLaboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Aix-Marseille Université, Unité Mixte de Recherche 7265, BIAM, Commissariat à l'Energie Atomique Cadarache, 13115 Saint-Paul-lez-Durance, France jean.alric@cea.ORCID 0000-0003-3574-2234
Aix-Marseille Université · FRUniversité Paris Cité · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Site-directed mutagenesis of chloroplast genes was developed three decades ago and has greatly advanced the field of photosynthesis research. Here, we describe a new approach for generating random chloroplast gene mutants that combines error-prone polymerase chain reaction of a gene of interest with chloroplast complementation of the knockout

Indexed as

MutagenesisBiolisticsChlamydomonas reinhardtiiChloroplast ProteinsCytochrome b6f ComplexGene Knockout TechniquesGene LibraryGenetic Complementation TestHydrophobic and Hydrophilic InteractionsPolymerase Chain ReactionStructure-Activity RelationshipChloroplast ProteinsCytochrome b6f ComplexpetD protein, cytochrome b(6)f complex

Identifiers

PMID29703866
PMCPMC6001340
OpenAlexW2803085211

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.