ArticleBMC genomics2018
Analysis of Theileria orientalis draft genome sequences reveals potential species-level divergence of the Ikeda, Chitose and Buffeli genotypes.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- From Benign Parasite to Emerging Pathogen: A Review ofAnimals : an open access journal from MDPI · 2026Review
- Advances and Challenges in the Diagnosis of Vector-Borne Protozoal Infections in Veterinary Medicine.Pathogens (Basel, Switzerland) · 2026Review
- Insights into the genetic diversity and population structure of prevalent Theileria orientalis in Bangladesh.PloS one · 2026Article
- Acute infection with the U.S. isolate ofFrontiers in veterinary science · 2026Article
- The efficacy of toltrazuril for prevention of oriental theileriosis (Theileria orientalis Ikeda genotype) in Friesian calves.Australian veterinary journal · 2025Article
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- Biting Back: Advances in Fighting Ticks and Understanding Tick-Borne Pathogens.Pathogens (Basel, Switzerland) · 2024Article
- Epidemiology and Transmission ofPathogens (Basel, Switzerland) · 2023Review
- Distribution ofPathogens (Basel, Switzerland) · 2022Article
- Characterisation of thePathogens (Basel, Switzerland) · 2022Article
- Complete Genomes ofPathogens (Basel, Switzerland) · 2022Article
- The Piroplasmida Babesia, Cytauxzoon, and Theileria in farm and companion animals: species compilation, molecular phylogeny, and evolutionary insights.Parasitology research · 2022Review
- Approaches to Integrated Parasite Management (IPM) forPathogens (Basel, Switzerland) · 2021Review
- Systematic Comparison of the Performances ofFrontiers in cellular and infection microbiology · 2021Article
- Comparative Genomics ofMicroorganisms · 2020Article
- Bovines Harbor a Diverse Array of Vector-Borne Pathogens in Northeast Algeria.Pathogens (Basel, Switzerland) · 2020Article
- Clinical Pathology, Immunopathology and Advanced Vaccine Technology in Bovine Theileriosis: A Review.Pathogens (Basel, Switzerland) · 2020Review
- A Targeted "Next-Generation" Sequencing-Informatic Approach to Define Genetic Diversity inPathogens (Basel, Switzerland) · 2020Article
- Theileria orientalis Ikeda Genotype in Cattle, Virginia, USA.Emerging infectious diseases · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundTheileria orientalis (Apicomplexa: Piroplasmida) has caused clinical disease in cattle of Eastern Asia for many years and its recent rapid spread throughout Australian and New Zealand herds has caused substantial economic losses to production through cattle deaths, late term abortion and morbidity. Disease outbreaks have been linked to the detection of a pathogenic genotype of T. orientalis, genotype Ikeda, which is also responsible for disease outbreaks in Asia. Here, we sequenced and compared the draft genomes of one pathogenic (Ikeda) and two apathogenic (Chitose, Buffeli) isolates of T. orientalis sourced from Australian herds.
resultsUsing de novo assembled sequences and a single nucleotide variant (SNV) analysis pipeline, we found extensive genetic divergence between the T. orientalis genotypes. A genome-wide phylogeny reconstructed to address continued confusion over nomenclature of this species displayed concordance with prior phylogenetic studies based on the major piroplasm surface protein (MPSP) gene. However, average nucleotide identity (ANI) values revealed that the divergence between isolates is comparable to that observed between other theilerias which represent distinct species. Analysis of SNVs revealed putative recombination between the Chitose and Buffeli genotypes and also between Australian and Japanese Ikeda isolates. Finally, to inform future vaccine studies, dN/dS ratios and surface location predictions were analysed. Six predicted surface protein targets were confirmed to be expressed during the piroplasm phase of the parasite by mass spectrometry.
conclusionsWe used whole genome sequencing to demonstrate that the T. orientalis Ikeda, Chitose and Buffeli variants show substantial genetic divergence. Our data indicates that future researchers could potentially consider disease-associated Ikeda and closely related genotypes as a separate species from non-pathogenic Chitose and Buffeli.
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Registered trials
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