Evidence map›Paper›PMID 29700986›Full record

ArticleAdvanced healthcare materials2018

Hydrophobic Patterning-Based 3D Microfluidic Cell Culture Assay.

Sewoon Han, Junghyun Kim, Rui Li, Alice Ma, Vincent Kwan, Kevin Luong, Lydia L Sohn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sewoon HanThe California Institute for Quantitative Biosciences, Stanley Hall, University of California, Berkeley, Berkeley, CA, 94720, USA.
Junghyun KimDepartment of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.ORCID 0000-0002-1385-481X
Rui LiDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Alice MaDepartment of Integrative Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Vincent KwanDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Kevin LuongDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.
Lydia L SohnDepartment of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.

Funding

Isolating Circulating Tumor CellsR21CA182375 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI SOHN, LYDIA L · 2015 to 2017
$605k
NCI NIH HHS R21 CA182375
6 · The paper itself

Abstract

Engineering physiologically relevant in vitro models of human organs remains a fundamental challenge. Despite significant strides made within the field, many promising organ-on-a-chip models fall short in recapitulating cellular interactions with neighboring cell types, surrounding extracellular matrix (ECM), and exposure to soluble cues due, in part, to the formation of artificial structures that obstruct >50% of the surface area of the ECM. Here, a 3D cell culture platform based upon hydrophobic patterning of hydrogels that is capable of precisely generating a 3D ECM within a microfluidic channel with an interaction area >95% is reported. In this study, for demonstrative purposes, type I collagen (COL1), Matrigel (MAT), COL1/MAT mixture, hyaluronic acid, and cell-laden MAT are formed in the device. Three potential applications are demonstrated, including creating a 3D endothelium model, studying the interstitial migration of cancer cells, and analyzing stem cell differentiation in a 3D environment. The hydrophobic patterned-based 3D cell culture device provides the ease-of-fabrication and flexibility necessary for broad potential applications in organ-on-a-chip platforms.

Indexed as

Cell Culture TechniquesLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesCell DifferentiationCell MovementHumansMCF-7 Cells3D cell culture model3D endothelium modelmicrofluidicsorgan-on-a-chip

Identifiers

PMID29700986
PMCPMC6342489

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.