ArticleNature protocols2018
Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Environment signal dependent biocontainment systems for engineered organisms: Leveraging triggered responses and combinatorial systems.Synthetic and systems biotechnology · 2025Review
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Newly Developed Electrochemiluminescence Based on Bipolar Electrochemistry for Multiplex Biosensing Applications: A Consolidated Review.Biosensors · 2023Review
- Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-β signaling.Cell reports · 2023Article
- Optogenetic approaches in biotechnology and biomaterials.Trends in biotechnology · 2022Review
- Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model.Nature communications · 2022Article
- Nuclear Localization Signals for Optimization of Genetically Encoded Tools in Neurons.Frontiers in cell and developmental biology · 2022Article
- Optogenetic Application to Investigating Cell Behavior and Neurological Disease.Frontiers in cellular neuroscience · 2022Review
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- A guide to the optogenetic regulation of endogenous molecules.Nature methods · 2021Review
- Cellular feedback dynamics and multilevel regulation driven by the hippo pathway.Biochemical Society transactions · 2021Review
- Single-component near-infrared optogenetic systems for gene transcription regulation.Nature communications · 2021Article
- Dual Systems for Enhancing Control of Protein Activity through Induced Dimerization Approaches.Advanced biology · 2021Review
- The frontier of live tissue imaging across space and time.Cell stem cell · 2021Review
- Advances in engineering near-infrared luminescent materials.iScience · 2021Review
- Two-photon conversion of a bacterial phytochrome.Biophysical journal · 2021Article
- Bacterial Phytochrome as a Scaffold for Engineering of Receptor Tyrosine Kinases Controlled with Near-Infrared Light.Journal of molecular biology · 2020Article
- Optogenetic regulation of endogenous proteins.Nature communications · 2020Article
- Near-infrared aggregation-induced enhanced electrochemiluminescence from tetraphenylethylene nanocrystals: a new generation of ECL emitters.Chemical science · 2019Article
- Light-Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Near-infrared (NIR, 740-780 nm) optogenetic systems are well-suited to spectral multiplexing with blue-light-controlled tools. Here, we present two protocols, one for regulation of gene transcription and another for control of protein localization, that use a NIR-responsive bacterial phytochrome BphP1-QPAS1 optogenetic pair. In the first protocol, cells are transfected with the optogenetic constructs for independently controlling gene transcription by NIR (BphP1-QPAS1) and blue (LightOn) light. The NIR and blue-light-controlled gene transcription systems show minimal spectral crosstalk and induce a 35- to 40-fold increase in reporter gene expression. In the second protocol, the BphP1-QPAS1 pair is combined with a light-oxygen-voltage-sensing (LOV) domain-based construct into a single optogenetic tool, termed iRIS. This dual-light-controllable protein localization tool allows tridirectional protein translocation among the cytoplasm, nucleus and plasma membrane. Both procedures can be performed within 3-5 d. Use of NIR light-controlled optogenetic systems should advance basic and biomedical research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.