ArticlePloS one2018
Profiling of epidermal lipids in a mouse model of dermatitis: Identification of potential biomarkers.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Autophagy-dependent changes in the sebaceous gland and epidermal lipid distribution localized by mass spectrometric imaging.JID innovations : skin science from molecules to population health · 2026Article
- Conventional Culture Conditions Limit the Ability of Bovine Mammary Epithelial Cells to Produce Milk Fat.Foods (Basel, Switzerland) · 2026Article
- Aging Effects on Lipid Metabolism in Response to Traumatic Brain Injury.Journal of neurochemistry · 2025Article
- Lipids potentially contribute to exacerbated inflammatory markers in Metabolic Syndrome mice acutely following pulmonary nanoparticle exposure.Journal of toxicology and environmental health. Part A · 2025Article
- Gut bacterial sphingolipid production modulates dysregulated skin lipid homeostasis.bioRxiv : the preprint server for biology · 2024Article
- The alpha-1A adrenergic receptor regulates mitochondrial oxidative metabolism in the mouse heart.Journal of molecular and cellular cardiology · 2024Article
- Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Step-by-Step Approach to Build Multiple Reaction Monitoring (MRM) Profiling Instrument Acquisition Methods for Class-based Lipid Exploratory Analysis by Mass Spectrometry.Journal of biomolecular techniques : JBT · 2023Article
- Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Suspect screening of exogenous compounds using multiple reaction screening (MRM) profiling in human urine samples.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2022Article
- Skeletal muscle undergoes fiber type metabolic switch without myosin heavy chain switch in response to defective fatty acid oxidation.Molecular metabolism · 2022Article
- Factorial Design to Optimize Matrix Spraying Parameters for MALDI Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2021Article
- Local and Systemic Changes in Lipid Profile as Potential Biomarkers for Canine Atopic Dermatitis.Metabolites · 2021Article
- A novel experimental workflow to determine the impact of storage parameters on the mass spectrometric profiling and assessment of representative phosphatidylethanolamine lipids in mouse tissues.Analytical and bioanalytical chemistry · 2021Article
- Octanoate is differentially metabolized in liver and muscle and fails to rescue cardiomyopathy in CPT2 deficiency.Journal of lipid research · 2021Article
- Loss of Muscle Carnitine Palmitoyltransferase 2 Prevents Diet-Induced Obesity and Insulin Resistance despite Long-Chain Acylcarnitine Accumulation.Cell reports · 2020Article
- Cannabidiol-Mediated Changes to the Phospholipid Profile of UVB-Irradiated Keratinocytes from Psoriatic Patients.International journal of molecular sciences · 2020Article
- Dietary ceramide 2-aminoethylphosphonate, a marine sphingophosphonolipid, improves skin barrier function in hairless mice.Scientific reports · 2020Article
- Article
- Exacerbation of Nanoparticle-Induced Acute Pulmonary Inflammation in a Mouse Model of Metabolic Syndrome.Frontiers in immunology · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Lipids are important structural and functional components of the skin. Alterations in the lipid composition of the epidermis are associated with inflammation and can affect the barrier function of the skin. SHARPIN-deficient cpdm mice develop a chronic dermatitis with similarities to atopic dermatitis in humans. Here, we used a recently-developed approach named multiple reaction monitoring (MRM)-profiling and single ion monitoring to rapidly identify discriminative lipid ions. Shorter fatty acyl residues and increased relative amounts of sphingosine ceramides were observed in cpdm epidermis compared to wild type mice. These changes were accompanied by downregulation of the Fasn gene which encodes fatty acid synthase. A profile of diverse lipids was generated by fast screening of over 300 transitions (ion pairs). Tentative attribution of the most significant transitions was confirmed by product ion scan (MS/MS), and the MRM-profiling linear intensity response was validated with a C17-ceramide lipid standard. Relative quantification of sphingosine ceramides CerAS(d18:1/24:0)2OH, CerAS(d18:1/16:0)2OH and CerNS(d18:1/16:0) discriminated between the two groups with 100% accuracy, while the free fatty acids cerotic acid, 16-hydroxy palmitic acid, and docosahexaenoic acid (DHA) had 96.4% of accuracy. Validation by liquid chromatography tandem mass spectrometry (LC-MS/MS) of the above-mentioned ceramides was in agreement with MRM-profiling results. Identification and rapid monitoring of these lipids represent a tool to assess therapeutic outcomes in SHARPIN-deficient mice and other mouse models of dermatitis and may have diagnostic utility in atopic dermatitis.
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